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Trizma® hydrochloride buffer solution

BioUltra, for molecular biology, pH 7.4

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Synonym(s):
Tris(hydroxymethyl)aminomethane hydrochloride buffer solution
Beilstein:
3675235
MDL number:
MFCD00012590
eCl@ss:
32129211
PubChem Substance ID:
329770223
NACRES:
ND.02

grade

for molecular biology

Quality Level

100

product line

BioUltra

form

liquid

concentration

1.0 M tris-HCl

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

7.4±0.1 (25 °C)

density

1.05 g/mL at 20 °C

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.03
λ: 280 nm Amax: 0.04

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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Other Notes

Buffer component (SET buffer) in the preparation of RNA

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Isolation of RNA.
S L Berger et al.
Methods in enzymology, 180, 3-13 (1989-01-01)
Satish N Dighe et al.
ACS omega, 5(48), 30971-30979 (2020-12-17)
Cholinesterases (ChE) are well-known drug targets for the treatment of Alzheimer's disease (AD). In continuation of work to develop novel cholinesterase inhibitors, we utilized a structure-based scaffold repurposing approach and discovered six novel ChE inhibitors from our recently developed DNA
Anna Iwaniak et al.
International journal of molecular sciences, 22(6) (2021-04-04)
In silico and in vitro methods were used to analyze ACE- and DPP-IV-inhibiting potential of Gouda cheese with a modified content of β-casein. Firstly, the BIOPEP-UWM database was used to predict the presence of ACE and DPP-IV inhibitors in casein

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