94334
Formononetin
analytical standard
Synonym(s):
7-Hydroxy-3-(4-methoxyphenyl)-4H-1-benzopyran-4-one, 7-Hydroxy-3-(4-methoxyphenyl)chromone, 7-Hydroxy-4′-methoxyisoflavone, Biochanin B, Daidzein 4′-methyl ether, Formonetin, Formononetol
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About This Item
Empirical Formula (Hill Notation):
C16H12O4
CAS Number:
Molecular Weight:
268.26
Beilstein:
237979
EC Number:
MDL number:
UNSPSC Code:
85151701
PubChem Substance ID:
NACRES:
NA.24
grade
analytical standard
Quality Level
Assay
≥98.0% (HPLC)
shelf life
limited shelf life, expiry date on the label
application(s)
food and beverages
format
neat
storage temp.
2-8°C
SMILES string
COc1ccc(cc1)C2=COc3cc(O)ccc3C2=O
InChI
1S/C16H12O4/c1-19-12-5-2-10(3-6-12)14-9-20-15-8-11(17)4-7-13(15)16(14)18/h2-9,17H,1H3
InChI key
HKQYGTCOTHHOMP-UHFFFAOYSA-N
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General description
Formononetin belongs to the isoflavones class of polyphenols.
Application
Formononetin has been used as a reference standard for the determination of formononetin in:
It may be used as a reference standard for the determination of formononetin in:
- Cow, goat, sheep and human milk samples by magnetic-micro-dispersive solid-phase extraction (m-μ-dSPE) combined with ultra-high performance liquid chromatography-triple quadrupole electrospray ionization-tandem mass spectrometry (UHPLC-QqQ-ESI-MS/MS) operating under the multiple reaction monitoring (MRM) mode of detection.
- Dairy products by quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction procedure and UHPLC-QqQ-ESI-MS/MS with MRM mode of detection.
It may be used as a reference standard for the determination of formononetin in:
- Natural products by turbulent flow chromatography liquid chromatography-high resolution mass spectrometry (TFC LC-HRMS).
- Soy-drinks by gas chromatography combined with MS/MS operating on electron ionization (EI) mode.
- Rat plasma by UHPLC-QqQ-ESI-MS/MS.
Biochem/physiol Actions
Isoflavone found in red clover. Effective against Giardia lamblia infection, at least partially by inducing detachment of trophozoites from intestinal mucosa.
Other Notes
Find a digital Reference Material for this product available on our online platform ChemisTwin® for NMR. You can use this digital equivalent on ChemisTwin® for your sample identity confirmation and compound quantification (with digital external standard). An NMR spectrum of this substance can be viewed and an online comparison against your sample can be performed with a few mouseclicks. Learn more here and start your free trial.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Development of liquid chromatography-tandem mass spectrometry method for analysis of polyphenolic compounds in liquid samples of grape juice, green tea and coffee.
Sapozhnikova Y.
Food Chemistry, 150, 87-93 (2014)
Direct analysis of prostaglandin-E2 and-D2 produced in an inflammatory cell reaction and its application for activity screening and potency evaluation using turbulent flow chromatography liquid chromatography-high resolution mass spectrometry
Shin JS, et al.
Journal of Chromatography A, 1463(7), 128-135 (2016)
Simultaneous determination of formononetin, calycosin and rhamnocitrin from Astragalus complanatus by UHPLC-MS-MS in rat plasma: application to a pharmacokinetic study.
Li Y, et al.
Journal of Chromatographic Science, 54(9), 1605-1612 (2016)
Multiresidue analysis of oestrogenic compounds in cow, goat, sheep and human milk using core-shell polydopamine coated magnetic nanoparticles as extraction sorbent in micro-dispersive solid-phase extraction followed by ultra-high-performance liquid chromatography tandem mass spectrometry
Socas-Rodriguez B, et al.
Analytical and Bioanalytical Chemistry, 410(7), 2031-2042 (2018)
Fast derivatization procedure for the analysis of phytoestrogens in soy milk by gas chromatography tandem mass spectrometry.
Benedetti B, et al.
Microchemical Journal, Devoted to the Application of Microtechniques in All Branches of Science, 137(7), 62-70 (2018)
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