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C0537

5-Carboxyfluorescein

Name: 5-Carboxyfluorescein
Brand: SIAL

≥99% purity (HPLC), powder

Synonym(s):

5-FAM

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About This Item

Empirical Formula (Hill Notation):

C₂₁H₁₂O₇

CAS Number:

76823-03-5

Molecular Weight:

376.32

NACRES:

NA.47

PubChem Substance ID:

24892258

UNSPSC Code:

12171500

MDL number:

MFCD00036874

Beilstein/REAXYS Number:

57037

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Product Name

5-Carboxyfluorescein, ≥99% (HPLC)

Quality Level

200

assay

≥99% (HPLC)

form

powder

solubility

0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

NJYVEMPWNAYQQN-UHFFFAOYSA-N

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Application

5-Carboxyfluorescein has been used as an internal marker in capillary isotachophoresis to quantify the charge-based low density lipoprotein subfractions.

Biochem/physiol Actions

5-Carboxyfluorescein is used for the staining of cells to differentiate between live and dead cells. It helps in monitoring the integrity and intactness of the cell membrane. It is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product 5-carboxyfluorescein. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Global Trade Item Number

SKU	GTIN
C0537-50MG	04061833289181

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