Skip to Content
Merck
CN

N4638

Neutral Red powder, BioReagent, cell culture | mammalian | 553-24-2

BioReagent, Powder

Synonym(s):

Neutral Red, 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
553-24-2
Molecular Weight:
288.78
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24897707
EC Number:
209-035-8
MDL number:
MFCD00012651
Beilstein/REAXYS Number:
3918740
Colour Index Number:
50040

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Neutral Red powder, BioReagent, cell culture | mammalian | 553-24-2, powder, BioReagent, suitable for cell culture

product line

BioReagent

Quality Level

200

form

powder

composition

Dye content, ≥90%

technique(s)

cell culture | mammalian: suitable, titration: suitable

color

brown to black

pH

6.8-8.0, red to yellow

mp

290 °C (dec.) (lit.)

solubility

H2O: 10 mg/mL

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

SMILES string

Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

Related Categories

General description

Neutral red (C.l. 50040, Cl Basic red 5) is a protonated diaminophenazine dye that has a small planar conjugated system.

Application

Neutral red has been used in several histological staining applications that include:
  • as a nuclear stain
  • as a counterstain in the Gram procedure, either alone, or as a component of the Twort stain, a fast green-neutral red mixture.
  • fluorescent staining of nucleic acids in nervous tissue, of lipids and hydrophobic substances such as suberin and of phenolic compounds in algal cell walls
  • detection of viable yeast cells in dandruff samples and of mussel larvae
  • Neutral red has also been used as a stain in flow cytometry.
Useful as an indicator for preparing neutral red paper, and as a biological stain.

Preparation Note

Purified

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000531014
0000531014
0000370047
0000343198
0000366296

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Pieter C Vogelaar et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 33(12), 2128-2138 (2018-04-17)
Mitochondrial dysfunction plays an important role in kidney damage in various pathologies, including acute and chronic kidney injury and diabetic nephropathy. In addition to the well-studied ischaemia/reperfusion (I/R) injury, hypothermia/rewarming (H/R) also inflicts acute kidney injury. Substituted 6-hydroxychromanols are a
Jung-Pyo Oh et al.
Molecular brain, 13(1), 112-112 (2020-08-18)
A unique feature of fear memory is its persistence that is highly relevant to fear and anxiety-related mental disorders. Recurrent reactivation of neural representations acquired from a traumatic event is thought to contribute to the indelibility of fear memory. Given
K R Sindhu et al.
Acta biomaterialia, 115, 197-209 (2020-08-20)
Hydrogels used in regenerative medicine are often designed to allow cellular infiltration, degradation, and neovascularization. Low molecular weight hydrogels (LMWHs), formed by self-assembly via non-covalent interactions, are gaining significant interest because they are soft, easy to use and injectable. We
Ryan Akin et al.
International journal of molecular sciences, 20(24) (2019-12-19)
Metastatic renal cell carcinoma (RCC) remains an important clinical issue; the 5-year survival rate of patients with metastasis is approximately 12%, while it is 93% in those with localized disease. There is evidence that blood cadmium and lead levels are
Mara Ceridono et al.
Regulatory toxicology and pharmacology : RTP, 63(3), 480-488 (2012-06-13)
This is the report from the "ECVAM-EFPIA workshop on 3T3 NRU Phototoxicity Test: Practical Experience and Implications for Phototoxicity Testing", jointly organized by ECVAM and EFPIA and held on the 25-27 October 2010 in Somma Lombardo, Italy. The European Centre
View All Related Papers

Global Trade Item Number

SKUGTIN
N4638-1G04061835573530
N4638-5G04061835573547

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service