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Merck
CN

P4272

3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt

Powder or crystals

Synonym(s):

5,5′-[3-(2-Pyridyl)-1,2,4-triazine-5,6-diyl]difuran-2-sulfonic acid disodium salt

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About This Item

Empirical Formula (Hill Notation):
C16H8N4Na2O8S2
CAS Number:
79551-14-7
Molecular Weight:
494.37
NACRES:
NA.47
PubChem Substance ID:
24898520
UNSPSC Code:
12171500
MDL number:
MFCD00040642
Beilstein/REAXYS Number:
5710932

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Product Name

3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt, Suitable for determination of Fe

Quality Level

200

form

powder or crystals

composition

Water: ≤ 13.0%

Carbon (Theory), anhydrous: 38.87%

Nitrogen (Theory), anhydrous: 11.33%

technique(s)

titration: suitable

color

white to dark yellow

solubility

water: 50 mg/mL, clear to slightly hazy

cation traces

C: 38.87% ((Theory), anhydrous), N: 11.33% ((Theory), anhydrous)

suitability

suitable for determination of iron

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]S(=O)(=O)c1ccc(o1)-c2nnc(nc2-c3ccc(o3)S([O-])(=O)=O)-c4ccccn4

InChI

1S/C16H10N4O8S2.2Na/c21-29(22,23)12-6-4-10(27-12)14-15(11-5-7-13(28-11)30(24,25)26)19-20-16(18-14)9-3-1-2-8-17-9;;/h1-8H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2

InChI key

IVLSEFOVPQFJBB-UHFFFAOYSA-L

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General description

3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt, also known as Ferene, is a chelating agent commonly used to measure the concentration of iron ions in aqueous solutions. The reagent is sensitive to ferrous iron ions (Fe2+) and forms a purple-colored complex with these ions in the presence of a reducing agent. The intensity of the purple color formed is proportional to the concentration of iron ions present in a solution and is quantified spectrophotometrically.

Application

3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt has been used as a component in a sensitive colorimetric assay (ferene-s assay) for the detection of iron. It has also been used as a ligand to study the complexation towards copper(II).

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN9759
BCCN9275
BCCN3166
BCCM6745
BCCL3263

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A facile colorimetric method for the quantification of labile iron pool and total iron in cells and tissue specimens.
Abbasi U, et al.
Scientific Reports, 6008, 11(1)-11(1) (2021)
Synthesis, Characterization, and Antimicrobial Activity of Novel Sulfonated Copper?Triazine Complexes.
Katugampala S, et al.
Bioinorganic Chemistry and Applications, 2530851-2530851 (2018)
Interaction between bovine control materials and the ferene iron method.
H M Barbour et al.
Annals of clinical biochemistry, 30 ( Pt 2), 221-222 (1993-03-01)
S Eskelinen et al.
Scandinavian journal of clinical and laboratory investigation, 43(5), 453-455 (1983-09-01)
Ferene-S was tested as the iron chromogen and the results were compared with the chromogen Ferrozine. With Ferene-S the volume of serum could be halved. We also tried to adapt Ferene-S in total iron binding capacity determinations but failed. From
D Krzyzanowska et al.
Biological chemistry, 382(7), 1027-1037 (2001-09-04)
It has been previously suggested that juvenile hormone binding protein(s) (JHBP) belongs to a new class of proteins. In the search for other protein(s) that may contain structural motifs similar to those found in JHBP, hemolymph from Galleria mellonella (Lepidoptera)
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Global Trade Item Number

SKUGTIN
P4272-5G04061834374091
P4272-25G04061832343907
P4272-1G04061834374084

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