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Merck
CN

P5504

Potassium phosphate dibasic trihydrate

ReagentPlus®, ≥99.0%

Synonym(s):

Dipotassium hydrogen phosphate trihydrate, Dipotassium phosphate, di-Potassium hydrogen phosphate trihydrate

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About This Item

Linear Formula:
K2HPO4 · 3H2O
CAS Number:
16788-57-1
Molecular Weight:
228.22
NACRES:
NA.25
PubChem Substance ID:
57654537
UNSPSC Code:
12161700
EC Number:
231-834-5
MDL number:
MFCD00149926

Key Documents

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Product Name

Potassium phosphate dibasic trihydrate, ReagentPlus®, ≥99.0%

InChI key

XQGPKZUNMMFTAL-UHFFFAOYSA-L

InChI

1S/2K.H3O4P.3H2O/c;;1-5(2,3)4;;;/h;;(H3,1,2,3,4);3*1H2/q2*+1;;;;/p-2

SMILES string

O.O.O.[K+].[K+].OP([O-])([O-])=O

product line

ReagentPlus®

assay

≥99.0%

form

powder or crystals

pH

9.2-9.4 (25 °C, 50 g/L)

solubility

water: 100 mg/mL, clear, colorless

storage temp.

room temp

Quality Level

200

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Application

Potassium phosphate dibasic trihydrate has been used in the preparation of potassium phosphate buffer for the resuspension of polyelectrolyte capsules. It has also been used as a buffer during glutaminase‑1 (GLS-1) enzyme assay and GLS-2 enzyme assay.

General description

Potassium phosphate solution have very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Potassium phosphate dibasic trihydrate is a key component of potassium phosphate buffer, phosphate-buffered saline (PBS), and phosphate-buffered EDTA (PBE). It is extensively used in the preparation of biological samples for various biological experiments. Potassium phosphate buffer is also used in methods such as equilibrium dialysis, high-performance liquid chromatography (HPLC), and affinity capillary electrophoresis (ACE) to study the characteristics of drug-protein interactions in the body. Potassium phosphate buffer solutions consist of mixtures of the potassium phosphate monobasic and potassium phosphate dibasic forms.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000513926
0000513926
0000501329
0000501328
0000501329

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Imidazolium-based Ionic Liquids as Adjuvants to Form Polyethylene Glycol with Salt Buffer Aqueous Biphasic Systems
Santos JHPM, et al.
Journal of Chemical and Engineering Data, 65(8), 3794?3801-3794?3801 (2020)
Daniel Valdepérez et al.
Journal of colloid and interface science, 474, 1-8 (2016-04-19)
Polyelectrolyte hollow capsules are versatile platforms typically used for encapsulation of a wide variety of macromolecules in their cavity. The polymer shell of these capsules as composed by alternating layers of oppositely charged polyelectrolytes also allows for adding additional functionalities.
Interaction between Potassium Phosphate Buffer Solution and Modeling Cell Membrane Investigated by Sum Frequency Generation Vibrational Spectroscopy
Weilai Y, et al.
Chinese Journal of Chemical Physics, 28(4), 518-524 (2015)
Antoine Desrut et al.
Journal of experimental botany, 71(22), 7301-7315 (2020-08-30)
Plants live in close relationships with complex populations of microorganisms, including rhizobacterial species commonly referred to as plant growth-promoting rhizobacteria (PGPR). PGPR are able to improve plant productivity, but the molecular mechanisms involved in this process remain largely unknown. Using
Michael J Soth et al.
Journal of medicinal chemistry, 63(21), 12957-12977 (2020-10-30)
Inhibition of glutaminase-1 (GLS-1) hampers the proliferation of tumor cells reliant on glutamine. Known glutaminase inhibitors have potential limitations, and in vivo exposures are potentially limited due to poor physicochemical properties. We initiated a GLS-1 inhibitor discovery program focused on
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