PPB010
Tris EDTA Buffer, pH 8.0
pHast Pack™, powder, 10X concentrate
Synonym(s):
10X TE buffer
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
Product Name
Tris EDTA Buffer, pH 8.0, pHast Pack™, powder, 10X concentrate
product line
pHast Pack™
form
powder
storage condition
(Tightly closed. Dry)
impurities
≤5 ppm Heavy Metals (lead)
color
white
pH
7.9—8.1
solubility
soluble, clear, colorless
cation traces
Fe: <10 ppm
foreign activity
DNAse, none detected
Nickase, none detected
Protease, none detected
RNAse, none detected
storage temp.
room temp
Application
TE buffer is intended for use in:
- Nucleic acid gel electrophoresis
- storage and dilution of DNA and RNA
- DNA automated sequencing
- Immunohistochemistry
Features and Benefits
- Fast and easy Ready-to-use buffer
- Minimizes effort - no need to weigh and adjust pH
- Saves time and eliminates exposure to hazardous chemicals
- Biological tests: free of DNase, RNase, protease, and nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
General description
10X Tris-EDTA or TE buffer has two components. Tris buffer is a commonly used stable, non-toxic, and non-hygroscopic buffer. Also, ethylenediaminetetraacetic acid (EDTA), a metal chelator, binds with cation metal ions that further inactivate the DNase enzyme which requires metal ions to activate. It has a pH range of 7.9 - 8.1.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Packaging
Foil pouch
Preparation Note
Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 10X solution containing 100mM Tris, 10mM EDTA, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Legal Information
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
signalword
Warning
hcodes
pcodes
Hazard Classifications
STOT RE 2 Inhalation
target_organs
Respiratory Tract
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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