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S0751

Sigma-Aldrich

Sodium phosphate monobasic

ReagentPlus®, ≥99.0%

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Synonym(s):
Monosodium dihydrogen orthophosphate, Monosodium phosphate, Sodium dihydrogen phosphate
Linear Formula:
NaH2PO4
CAS Number:
Molecular Weight:
119.98
EC Number:
MDL number:
eCl@ss:
38070208
PubChem Substance ID:

Quality Level

product line

ReagentPlus®

Assay

≥99.0%

form

powder or crystals

technique(s)

DNA extraction: suitable
electrophoresis: suitable
immunocytochemistry: suitable

pH

4.0-4.5 (25 °C, 50 g/L in water)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

water: 100 mg/mL, clear, colorless

application(s)

detection

SMILES string

[Na+].OP(O)([O-])=O

InChI

1S/Na.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1

InChI key

AJPJDKMHJJGVTQ-UHFFFAOYSA-M

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General description

Sodium phosphate monobasic acts as an acidulant in laxatives and effervescent powders. It is used in beverages, cheese and soft drink formulations.

Application

Sodium phosphate monobasic has been used:
  • as a reagent for virus transfection and fiber implantation in mouse
  • in phosphate buffer for immunocytochemistry and to detect individual catalase activity by PAGE (polyacrylamide gel electrophoresis)
  • as a buffer for cycling assay
  • in automated DNA extraction method
  • as a chemical in artificial cerebro-spinal fluid (aCSF) solution for the preparation of brainstem-spinal cord from rats

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Dictionary of Food ingredients (2001)
In vitro Brainstem-spinal Cord Preparation from Newborn Rat
Le Gal JP, et al.
The Journal of Neuroscience (2016)
Two Different Methods of Quantification of Oxidized Nicotinamide Adenine Dinucleotide (NAD+) and Reduced Nicotinamide Adenine Dinucleotide (NADH) Intracellular Levels: Enzymatic Coupled Cycling Assay and Ultra-performance Liquid Chromatography (UPLC)-Mass Spectrometry
Kanamori KS, et al.
Bio-protocol, 8(14) (2018)
Automated and manual methods of DNA extraction for Aspergillus fumigatus and Rhizopus oryzae analyzed by quantitative real-time PCR
Francesconi A, et al.
Journal of Clinical Microbiology, 46(6), 1978-1984 (2008)
Detection of Catalase Activity by Polyacrylamide Gel Electrophoresis (PAGE) in Cell Extracts from Pseudomonas aeruginosa
Pezzoni M, et al.
Microbiology (2016)

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