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S7907

Sigma-Aldrich

Sodium phosphate dibasic

BioXtra, ≥99.0%

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Synonym(s):
sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate
Linear Formula:
Na2HPO4
CAS Number:
7558-79-4
Molecular Weight:
141.96
EC Number:
231-448-7
MDL number:
MFCD00003496
eCl@ss:
38070211
PubChem Substance ID:
24899769
NACRES:
NA.25

product line

BioXtra

Quality Level

200

Assay

≥99.0%

form

powder or crystals

impurities

≤0.1% Insoluble matter

pH

8.0-11.0 (20 °C, 1 M in H2O)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.002%
Cu: ≤0.0005%
Fe: ≤0.002%
K: ≤0.005%
Mg: ≤0.005%
NH4+: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%

absorption

<0.05 at 260 in H2O at 1 M
<0.05 at 280 in H2O at 1 M

SMILES string

[Na+].[Na+].[H]OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

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General description

Sodium phosphate dibasic (NaH2PO4), also known as disodium phosphate, is a commonly used phosphate salt in biochemistry research due to its versatile properties. It is a key component in the preparation of phosphate buffer saline (PBS) and phosphate buffer, a commonly used buffer solution in many biological applications. In addition to its buffer properties, NaH2PO4 is also used as a precipitating agent to form silver orthophosphate (Ag3PO4) powders, making it a valuable tool in many laboratory settings. Disodium phosphate is also a widely used emulsifier in dairy products such as cheese and other foods.

Application

Sodium phosphate dibasic has been used:
  • as a component of phosphate buffered saline (PBS)
  • as a component of buffer for the preparation of cryopreservation diluent
  • in the preparation of McIlvaine buffer (citrate-phosphate buffer) for toluidine blue staining
  • in the preparation of the S9 mix for the Ames test, and SOS Chromotest
  • in the preparation of calcium deficient hydroxyapatite (CDHA) paste during the fabrication process of the CDHA scaffold

Features and Benefits

  • The BioXtra product line ensures high-quality products for consistent results.
  • High level of assay ( ≥99.0%), indicating its purity.
  • Powder form for easy handling and use.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Sperm protamine-status correlates to the fertility of breeding bulls
Dogan S, et al.
Biology of Reproduction, 92(4) (2015)
Effect of Emulsifier Salts on Textural and Flavor Properties of Processed Cheeses
Gupta SK, et al.
Journal of Dairy Science, 67(4), 764-778 (1984)
Whole-mount Immunohistochemistry of Adult Zebrafish Retina for Advanced Imaging
Nagashima M and Hitchcock PF
Bio-protocol, 10(24) (2020)
Effect of phosphate salts (Na3PO4, Na2HPO4, and NaH2PO4) on Ag3PO4 morphology for photocatalytic dye degradation under visible light and toxicity of the degraded dye products
Pongsaton A, et al.
Industrial & Engineering Chemistry Research, 52(49), 17369-17173 (2013)
Sequential generations of honey bee (Apis mellifera) queens produced using cryopreserved semen
Hopkins BK, et al.
Reproduction, Fertility, and Development, 24(8), 1079-1083 (2012)
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