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About This Item
UNSPSC Code:
41106514
Biological source:
human prostate
Relevant disease(s):
cancer
Growth mode:
Adherent
Morphology:
Cobblestone
Receptors:
CD44, alpha2 integrin, alpha6 integrin, EGFR, FGFR1, LIFR
Technique(s):
cell culture | mammalian: suitable
biological source
human prostate
form
liquid
packaging
tube of 5 μg 10021101-DNA-5UG, pkg of vial of cells 10021101-1VL
growth mode
Adherent
karyotype
4n (67 to 88)
morphology
Cobblestone
products
More differentiated than Bob with increased CD24, PAP, PSCA expression (prostate differentiation markers). 10% 3-D sphere formation in Matrigel. Migratory and invasive when compared to benign prostate tumour. More invasive than Bob.
receptors
CD44, alpha2 integrin, alpha6 integrin, EGFR, FGFR1, LIFR
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Quality Level
Application
Prostate cancer research including drug testing.
Biochem/physiol Actions
First spontaneously immortalized prostate cancer cell line to be established from a trans-rectal needle biopsy (TRBP) of a patient with castration-resistant prostate cancer (CRPC). Bob is a novel pre-clinical model for functional studies in CRPC and especially for studying the CRPC "basal" phenotype. SerBob grows as a monolayer culture with limited differentiation. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Human prostate cancer spontaneously immortalized
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,14
D16S539: 10,12
D5S818: 13
D7S820: 10,11
THO1: 9
TPOX: 8,10
vWA: 17
CSF1PO: 11,12
D13S317: 9,14
D16S539: 10,12
D5S818: 13
D7S820: 10,11
THO1: 9
TPOX: 8,10
vWA: 17
Packaging
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Preparation Note
Keratinocyte-SFM (Invitrogen, catalogue no 37010-022, supplied with prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53), bovine pituitary extract (BPE) and glutamine) + 2ng/ml leukaemia inhibitory factor + 2ng/ml stem cell factor + 100ng/ml cholera toxin + 1ng/ml granulocyte macrophage colony stimulating factor.
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. At confluence 5-7 x 104 cells/cm2 can be expected.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Gerhardt Attard et al.
The Prostate, 69(14), 1507-1520 (2009-06-23)
New in vitro models of castration-resistant prostate cancer (CRPC) are urgently required. Trans-rectal needle biopsies (TRBP) of the prostate were performed for research purposes on progressing CRPC patients who had not received prior treatment to the prostate. Biopsies were immediately
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