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Merck
CN

137269

BIS-TRIS propane

EMPROVE® EVOLVE

Synonym(s):

BIS-TRIS propane, 1,3-Bis[tris(hydroxymethyl)methylamino]propane

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About This Item

Linear Formula:
CH2[CH2NHC(CH2OH)3]2
CAS Number:
64431-96-5
Molecular Weight:
282.33
UNSPSC Code:
12161705
Beilstein/REAXYS Number:
1786109
MDL number:
MFCD00004689

Key Documents

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Product Name

BIS-TRIS propane, EMPROVE® EVOLVE

InChI key

HHKZCCWKTZRCCL-UHFFFAOYSA-N

SMILES string

OCC(CO)(CO)NCCCNC(CO)(CO)CO

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

biological source

synthetic

product line

EMPROVE® EVOLVE

form

powder

technique(s)

cell culture | mammalian: suitable

pH

6.3-9.5

useful pH range

6.3-9.5

pKa (25 °C)

(1) 6.8, (2) 9.0

pKa 

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)
164-165 °C (lit.)

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

storage temp.

2-30°C

Quality Level

400

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Application

Bis Tris Propane is a unique biological buffer also referred to as a “Universal” buffer, which means the buffer capacity covers a wide range. Bis Tris Propane has two pKas of 6.3 and 9.5, which expands the buffering range from 5.0-10.5-making this a versatile buffer for biological systems. Bis Tris Propane is considered to be non-toxic to culture cell lines and provides high-solution clarity.A review of DNA polymerase techniques lists bis tris propane as a suitable buffer for PCR methods as a universal buffer pH gradient is employed.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000514139
0000514139
0000492777
0000492777
0000454412

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