Skip to Content
Merck
CN

Key Documents

View All Documentation

137269

BIS-TRIS propane

EMPROVE® EVOLVE

Synonym(s):

BIS-TRIS propane, 1,3-Bis[tris(hydroxymethyl)methylamino]propane

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Linear Formula:
CH2[CH2NHC(CH2OH)3]2
CAS Number:
64431-96-5
Molecular Weight:
282.33
UNSPSC Code:
12161705
Beilstein/REAXYS Number:
1786109
MDL number:
MFCD00004689
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

synthetic

Quality Segment

400

product line

EMPROVE® EVOLVE

form

powder

technique(s)

cell culture | mammalian: suitable

pH

6.3-9.5

useful pH range

6.3-9.5

pKa (25 °C)

(1) 6.8, (2) 9.0

pKa 

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

storage temp.

2-30°C

SMILES string

OCC(CO)(CO)NCCCNC(CO)(CO)CO

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

InChI key

HHKZCCWKTZRCCL-UHFFFAOYSA-N

Application

Bis Tris Propane is a unique biological buffer also referred to as a “Universal” buffer, which means the buffer capacity covers a wide range. Bis Tris Propane has two pKas of 6.3 and 9.5, which expands the buffering range from 5.0-10.5-making this a versatile buffer for biological systems. Bis Tris Propane is considered to be non-toxic to culture cell lines and provides high-solution clarity.A review of DNA polymerase techniques lists bis tris propane as a suitable buffer for PCR methods as a universal buffer pH gradient is employed.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Still not finding the right product?

Explore all of our products under BIS-TRIS propane

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Stay compliant with GMP using the Emprove® Program

Accelerate qualification, maintain compliance, and save time with Emprove® Dossiers.

Download Dossiers

Note: you will be brought to EmproveSuite.com. Your username and password are separate from SigmaAldrich.com and may differ.

Register for free and access Emprove® Dossiers within minutes. Upgrade your subscription plan to unlock advanced and customized content in the Emprove® Suite

Not finding what you are looking for? Visit the Emprove® Program Help page.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Global Trade Item Number

SKUGTIN
PH016042-250MG04061831282450
137269901004065267204826
137269100004065267204802
137269500004065267204819
137269010004065267204796
137269000304065267204789