Skip to Content
Merck
CN

137270

HEPES

EMPROVE® EVOLVE

Synonym(s):

HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
7365-45-9
Molecular Weight:
238.30
UNSPSC Code:
12161700
Beilstein/REAXYS Number:
883043
MDL number:
MFCD00006158

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

HEPES, EMPROVE® EVOLVE

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

biological source

synthetic

product line

EMPROVE® EVOLVE

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals; tested

pH

4.7-5.5

useful pH range

6.8-8.2
6.8-8.2

pKa (25 °C)

7.5

pKa 

7.5

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

storage temp.

2-30°C

Quality Level

400

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

HEPES is a biological buffer often referred to as a “Good′s” buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high solution clarity.HEPES is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.

Packaging

100 g in poly bottle
5, 10, 25, 50 kg in poly drum

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000503713
0000506614
0000506614
0000503713
0000414404

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service