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Merck
CN

36098

Anti-Rabbit IgG F(ab′)2 fragment - Atto 488, whole molecule antibody produced in goat

1 mg/mL protein

Synonym(s):

Atto 488-[F(ab′)2 fragment goat-Anti-rabbit IgG], whole molecule

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About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
Atto 488 conjugate
Clone:
polyclonal
Application:
Species reactivity:
rabbit
Citations:
2

Key Documents

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Product Name

Anti-Rabbit IgG F(ab′)2 fragment - Atto 488, whole molecule antibody produced in goat, 1 mg/mL protein

conjugate

Atto 488 conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

contains

50% glycerol as stabilizer

species reactivity

rabbit

concentration

1 mg/mL protein

fluorescence

λex 500 nm; λem 522 nm in PBS

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Related Categories

Analysis Note

Unconjugated dye ≤5% of total fluorescence; dye-to protein ratio ≥2

Application

Atto 488-goat anti-rabbit IgG F(ab′)2 fragment, whole molecule antibody can be used for fluorescence-based immunoassays.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-rabbit IgGs associate with rabbit IgGs.

Immunogen

rabbit IgG F(ab′)2 fragment

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Physical form

Atto 488 F(ab)′2 fragment goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1mg/mL solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Preparation Note

F(ab)′2 fragment goat anti-rabbit is conjugated to Atto 488 NHS and purified by gel permeation chromatography and dialysis to remove unbound dye.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
BCCK5553
BCCC5101
BCBX9837
BCBS2543V
BCBP7754V

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Shiqi Ma et al.
Neoplasia (New York, N.Y.), 21(4), 343-352 (2019-03-05)
There are seven ligands for the epidermal growth factor receptor (EGFR) ErbB1 and two ligands for ErbB3. EGFR can form a homodimer or a heterodimer with ErbB3. In this study, we investigated whether homodimers or heterodimers, and which ligand, play
Janosch P Heller et al.
Methods in molecular biology (Clifton, N.J.), 1938, 69-84 (2019-01-09)
In recent years it has become apparent that astroglia are not only essential players in brain development, homeostasis, and metabolic support but are also important for the formation and regulation of synaptic circuits. Fine astrocytic processes that can be found in

Articles

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

使用抗体 Atto 染料缀合物进行荧光多重检测

免疫印迹（蛋白质印迹转印）是现代蛋白质组学研究中的常用技术。

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