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Merck
CN

54457

HEPES

≥99.5% (titration), powder, for molecular biology, BioUltra

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
7365-45-9
Molecular Weight:
238.30
NACRES:
NA.25
PubChem Substance ID:
57651473
UNSPSC Code:
12161700
EC Number:
230-907-9
MDL number:
MFCD00006158
Beilstein/REAXYS Number:
883043

Key Documents

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Product Name

HEPES, BioUltra, Molecular Biology, ≥99.5% (T)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

grade

Molecular Biology

product line

BioUltra

assay

≥99.5% (T)

form

powder

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

ign. residue

≤0.1% (as SO4)

loss

≤0.5% loss on drying, 110 °C

pH

5.0-6.5 (25 °C, 1 M in H2O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.045
λ: 280 nm Amax: 0.035

Quality Level

200

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Application

HEPES has been used as a component of:
  • DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells
  • RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)
  • Recapture solution to re-capture RNA exosomes from the glycerol gradient
  • ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation
  • 2X HBS buffer that is used in transfection process for the production of recombinant lentivectors

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Other Notes

3′-Terminal labeling of RNA with T4 RNA ligase; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer.
Easily compare specifications for HEPES products with the HEPES specification table.

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM9649
BCCL8226
BCCK3478
BCCH8168
BCCJ2320

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The correction of the metabolic parameters of msg-induced obesity in rats by 2-[4-(benzyloxy) phenoxy] acetic acid.
Konopelniuk V, et al.
Journal of Nutrition and Intermediary Metabolism, 13, 1-9 (2018)
Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures.
Zhang F, et al.
Nature Protocols, 5(3), 439-439 (2010)
Sample Preparation of Telomerase Subunits for Crystallization.
Huang J, et al.
Bio-protocol, 5(16) (2015)
RNA Degradation Assay Using RNA Exosome Complexes, Affinity-purified from HEK-293 Cells.
Domanski M and LaCava J
Bio-protocol, 7(8) (2017)
Isolation and analysis of nuclear RNA.
J R Nevins
Methods in enzymology, 152, 234-241 (1987-01-01)
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