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Sigma-Aldrich

HEPES buffer solution

1 M in H2O

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Synonym(s):
HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
CAS Number:
7365-45-9
MDL number:
MFCD00006158
eCl@ss:
32129211
PubChem Substance ID:
57652951
NACRES:
NA.25

form

liquid

Quality Level

100

concentration

1 M in H2O
238 g/L HEPES in H2O

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

5.5±0.5

useful pH range

6.8-8.2

density

1.07 g/mL at 20 °C

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
  • As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
  • To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
  • As a component of dissection buffer for culturing rat cortical neurons
  • Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
  • As a component of Danieu′s aquarium water used in agarose gel preparation

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Cultivation of Salmonella enterica serovar Typhimurium in a norepinephrine-containing medium alters in vivo tissue prevalence in swine.
Toscano MJ, et al.
Journal of experimental animal science, 43 , 329-338 (2007)
Phagocytosis Assay of Microglia for Dead Neurons in Primary Rat Brain Cell Cultures.
Zhao X, et al.
Bio-protocol, 6(8) (2016)
Group 2 innate lymphoid cells express functional NKp30 receptor inducing type 2 cytokine production.
Salimi M, et al.
Journal of Immunology, 196(1), 45-54 (2016)
"Hypoxia-induced metastasis model in embryonic zebrafish.
Rouhi P, et al.
Nature Protocols, 5(12), 1911-1911 (2010)
Using mammalian GFP reconstitution across synaptic partners (mGRASP) to map synaptic connectivity in the mouse brain.
Feng L, et al.
Nature Protocols, 9(10), 2425-2425 (2014)
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