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Merck
CN

93290

TRIS borate – EDTA buffer solution

for Northern and Southern blotting, solution

Synonym(s):

TBE buffer

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25
eCl@ss:
32129211

Key Documents

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Product Name

TRIS borate – EDTA buffer solution, for Northern and Southern blotting, solution

pH

8.6-9.0

grade

Molecular Biology

description

long run, concentrate

product line

BioReagent

form

solution

quality

10× concentrate
ready-to-use solution

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

storage temp.

room temp

Quality Level

100

Related Categories

Application

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.

General description

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. The TBE solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).

Preparation Note

The TBE 10x liquid contains 1.3M TRIS, 450 mM boric acid and 25 mM EDTA in water.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H360FD

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM6037
BCCK0303
BCCH2964
BCCD2319
BCCB9874

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L. Johnston-Dow et al.
Biotechniques, 5, 754-754 (1987)

Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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