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Trizma^® base

Name: Trizma<SUP>®</SUP> base
Brand: SIGMA

BioUltra, for molecular biology, ≥99.8% (T)

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Synonym(s):

2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

Linear Formula:

NH₂C(CH₂OH)₃

CAS Number:

77-86-1

Molecular Weight:

121.14

Beilstein:

741883

EC Number:

201-064-4

MDL number:

MFCD00004679

PubChem Substance ID:

57653540

NACRES:

NA.25

grade

for molecular biology

Quality Level

200

description

aminopeptidase substrate

product line

BioUltra

Assay

≥99.8% (T)

form

crystalline

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

ign. residue

≤0.01% (as SO₄)

loss

≤0.5% loss on drying, 110 °C

color

colorless to white

pH

10.5-12.0 (25 °C, 4 M in H₂O)

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)
168-172 °C

solubility

H₂O: 4 M at 20 °C, clear, colorless

anion traces

chloride (Cl^-): ≤20 mg/kg
sulfate (SO₄^2-): ≤5 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string

NC(CO)(CO)CO

λ

4 M in H₂O

UV absorption

λ: 260 nm A_max: 0.10
λ: 280 nm A_max: 0.08

suitability

in accordance for luminescence

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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Related Categories

Biological Buffers

High-Purity Biochemicals and Reagents

Tris

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

The BioChemika quality is for the usual biochemical applications. The products designated as BioChemika Ultra grade are suitable for different applications like purification, precipitation, crystallization and other applications which require tight control of elemental content. Trace elemental analyses have been performed for all qualities. The molecular biology qualities are also tested for absence of nucleases and the luminescence quality spectroscopic tests are performed.

Application

Trizma has been used:

In the dissection medium and culture medium of Hippocampal organotypic tissue cultures
As a component of lysis buffer before immunoprecipitation and immunoblotting
As a component of harvest buffer, lysis buffer, ChIP dilution buffer, and wash buffer in chromatin immunoprecipitation method for studying protein-DNA binding
As a component of mixed buffer formulations
As a component of NuPAGE loading buffer for SDS-PAGE and as a component of transfer buffer for Western blotting

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma base is used as a general reagent for the preparation of all types of Tris buffers.

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Running buffer component in non-denaturing agarose gel electrophoresis; In non-denaturing and urea-denaturing polyacrylamide gel electrophoresis; DNA digest analysis with capillary electrophoresis.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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R.C. Ogden et al.

Methods in Enzymology, 152, 78-78 (1987)

A new method allowing long-term potentiation recordings in hippocampal organotypic slices.

Paci P, et al.

Brain and Behavior, 7(5), e00692-e00692 (2017)

In vitro Detection of S-acylation on Recombinant Proteins via the Biotin-Switch Technique.

Qi D and Innes RW

Bio-protocol, 4(22) (2014)

A fast, efficient chromatin immunoprecipitation method for studying protein-DNA binding in Arabidopsis mesophyll protoplasts.

Plant methods, 13(1), 42-42 (2017)

Sambrook J and Russell DW

Molecular Cloning: A Laboratory Manual (2001)

View All Related Papers

Protocols

Restriction Enzyme Cloning Manual Buffer Recipes

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

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