Skip to Content
Merck
CN

Key Documents

View All Documentation

93363

Trizma® hydrochloride

BioUltra, Molecular Biology, ≥99.0% (AT)

Synonym(s):

TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
1185-53-1
Molecular Weight:
157.60
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
57653541
EC Number:
214-684-5
Beilstein/REAXYS Number:
3675235
MDL number:
MFCD00012590
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

grade

Molecular Biology

Quality Level

200

product line

BioUltra

assay

≥99.0% (AT)

form

powder or crystals

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

ign. residue

≤0.2% (as SO4)

loss

≤0.2% loss on drying, 110 °C

pH

2.5-4.0 (25 °C, 4 M in H2O)

useful pH range

7.0-9.0

pKa (25 °C)

8.1

mp

150-152 °C

solubility

H2O: 4 M at 20 °C, clear, colorless

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

4 M in H2O

UV absorption

λ: 260 nm Amax: 0.12, λ: 280 nm Amax: 0.08

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma hydrochloride is used as a general reagent for the preparation of all types of Tris buffers.

Other Notes

Component of extraction buffer e.g. phenol extraction of DNA or RNA; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE.
Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCN6064
BCCN6314
BCCN0127
BCCM7499
BCCM1820

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Santiago Vilanova et al.
Plant methods, 16, 110-110 (2020-08-15)
The use of sequencing and genotyping platforms has undergone dramatic improvements, enabling the generation of a wealth of genomic information. Despite this progress, the availability of high-quality genomic DNA (gDNA) in sufficient concentrations is often a main limitation, especially for
D Sirieix et al.
American journal of respiratory and critical care medicine, 155(3), 957-963 (1997-03-01)
Metabolic acidosis induces a decrease in the developed force of cardiac muscle by affecting every step of the excitation--contraction coupling pathway. Due to transient worsening in intracellular acidosis, the value of administering sodium bicarbonate therapeutically during acute acidosis has been
G G Nahas et al.
Drugs, 55(2), 191-224 (1998-03-20)
THAM (trometamol; tris-hydroxymethyl aminomethane) is a biologically inert amino alcohol of low toxicity, which buffers carbon dioxide and acids in vitro and in vivo. At 37 degrees C, the pK (the pH at which the weak conjugate acid or base
Large- and small-scale phenol extractions.
D M Wallace
Methods in enzymology, 152, 33-41 (1987-01-01)
Aquiles Sales Craveiro Sarmento et al.
Biochimica et biophysica acta. Molecular and cell biology of lipids, 1865(4), 158610-158610 (2020-01-10)
CGL type 2 is a rare autosomal recessive syndrome characterized by an almost complete lack of body fat. CGL is caused by loss-of-function mutations in both alleles of the BSCL2 gene that codifies to seipin. Subjects often show hyperglycemia, decreased
View All Related Papers

Global Trade Item Number

SKUGTIN
93363-500G04061833282687
93363-100G04061833282670
93363-50G04061833282694

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service