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A4414

Albumin from porcine serum

lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

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About This Item

CAS Number:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
Porcine
Assay:
≥99% (agarose gel electrophoresis)
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
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biological source

Porcine

Quality Level

assay

≥99% (agarose gel electrophoresis)

form

lyophilized powder

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

Porcine ... ALB(396960)

General description

Albumin is present abundantly in serum. Porcine serum albumin (PSA) comprises a flexible C-terminal region compared to other albumins.

Application

Albumin from porcine serum has been used:
  • as a non-hazardous protein to test its effect on T84 polarized intestinal monolayers and Caco2 polarized intestinal monolayers barrier integrity in trans-Epithelial Electrical Resistance (TEER) assay
  • to test its effect on the human intestinal epithelial cell lines
  • to test its interaction with the biodegradable ester-functionalized gemini surfactant 16-E2-16 using ultraviolet (UV)-visible and circular dichroism (CD) spectroscopy measurements

Biochem/physiol Actions

Albumin binds to many blood associated proteins. Porcine serum albumin (PSA) is regarded as a major allergen. PSA is used in the immunosensors, molecularly imprinted nanogels (MIP-NGs) and enzyme-linked immunosorbent assay (ELISA) protein-based detection for assessing contamination, especially in meat products.

Preparation Note

Prepared from albumin (A2764).


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Chehasan Cheubong et al.
Analytical chemistry, 92(9), 6401-6407 (2020-04-14)
Accurate, simple, and valuable analytical methods for detection of food contamination are rapidly expanding to evaluate the validity of food product quality because of ethnic considerations and food safety. Herein molecularly imprinted nanogels (MIP-NGs), capable of porcine serum albumin (PSA)
An ester-functionalized cationic gemini surfactant mediated structural transitions of porcine serum albumin (PSA) via binding interaction
Akram M, et al.
Colloids and Surfaces. A, Physicochemical and Engineering Aspects, 516, 245-253 (2017)
Bryan P Hurley et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 92, 75-87 (2016-04-10)
Human intestinal epithelial cell lines (T84, Caco-2, and HCT-8) grown on permeable Transwell™ filters serve as models of the gastrointestinal barrier. In this study, this in vitro model system was evaluated for effectiveness at distinguishing between hazardous and non-hazardous proteins. Indicators



Global Trade Item Number

SKUGTIN
A4414-1G04061826740903
A4414-500MG04061833433300
A4414-5G04061833627686