B4882
Bromelain from pineapple stem
≥3 units/mg protein
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-387-5
MDL number:
Specific activity:
≥3 units/mg protein
form
lyophilized powder
Quality Level
specific activity
≥3 units/mg protein
composition
Protein, ≥30% biuret
storage temp.
−20°C
Application
Bromelain from pineapple stem has been used:
- to evaluate the aqueous micellar two-phase systems composed of ionic liquids as co-surfactants
- as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) and in cells proliferation assay
- to study its potential analgesic effect and to evaluate its hydrolyzingeffects on proenkephalin (PENK)-derived peptides containing enkephalin sequences
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .
Biochem/physiol Actions
Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product B4882 is from pineapple stem. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.
Preparation Note
The product may be suspended in acetate buffer, pH 4.5 at 1 mg/mL concentration, yielding a hazy, off-white suspension.
Other Notes
One unit will release 1.0 micromole of p-nitrophenol from N-alpha-CBZ-L-Lysine p-nitrophenyl ester per minute at pH 4.6 at 25 °C. One old titrimetric unit (pH 4.5 at 45 °C) is equivalent to approximately 1.7 new units (pH 4.6 at 25 °C).
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
低风险生物材料
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Protocols
Protocol for HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column
Soghra Khatun Haq et al.
European journal of biochemistry, 269(1), 47-52 (2002-01-11)
Equilibrium studies on the acid included denaturation of stem bromelain (EC 3.4.22.32) were performed by CD spectroscopy, fluorescence emission spectroscopy and binding of the hydrophobic dye, 1-anilino 8-naphthalene sulfonic acid (ANS). At pH 2.0, stem bromelain lacks a well defined
In vitro antiproliferative activity of fresh pineapple juices on ovarian and colon cancer cell lines
Gani MBA, et al.
International Journal of Peptide Research and Therapeutics, 21(3), 353-364 (2015)
Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants
Vicente FA, et al.
Process. Biochem., 51(4), 528-534 (2016)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B4882-100G | 04061833433492 |
| B4882-1KG | 04061833433508 |
| B4882-10G | 04061835553846 |
| B4882-500G | 04061833433515 |
| B4882-25G | 04061835553853 |