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Merck
CN

B6008

Ponceau BS

Dye content ~60 %, Powder

Synonym(s):

Acid Red 66, Biebrich scarlet WS, Biebrich scarlet sodium salt

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About This Item

Empirical Formula (Hill Notation):
C22H14N4Na2O7S2
CAS Number:
4196-99-0
Molecular Weight:
556.48
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24891888
EC Number:
224-084-5
MDL number:
MFCD00003891
Colour Index Number:
26905
Beilstein/REAXYS Number:
3883900

Key Documents

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Product Name

Ponceau BS, Dye content ≥55 %

form

powder

Quality Level

200

composition

Dye content, ≥55%

technique(s)

microbe id | staining: suitable

color

red to brown

solubility

ethanol: 1 mg/mL, H2O: >30 mg/mL

εmax

≥19000 at 510 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1ccc2ccccc2c1\N=N\c3ccc(cc3S([O-])(=O)=O)\N=N\c4ccc(cc4)S([O-])(=O)=O

InChI

1S/C22H16N4O7S2.2Na/c27-20-12-5-14-3-1-2-4-18(14)22(20)26-25-19-11-8-16(13-21(19)35(31,32)33)24-23-15-6-9-17(10-7-15)34(28,29)30;;/h1-13,27H,(H,28,29,30)(H,31,32,33);;/q;2*+1/p-2/b24-23+,26-25+;;

InChI key

VVAVKBBTPWYADW-RVTJCSDESA-L

Related Categories

Application

Ponceau BS has been used in Masson′s trichrome stain for the staining of collagen. It has also been used for eosinophil staining.

Biochem/physiol Actions

Ponceau BS is used in Masson′s trichrome staining procedure, along with acid fuchsin, for the labeling of collagen and muscle red.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS7903
SHBR4564
SHBQ3355
SHBP8595
SHBN4821

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Interleukin-4 has basophilic and eosinophilic cell growth-promoting activity on cord blood cells.
Favre C, et al.
Blood, 75, 67-67 (1990)
Shariff S and Kaler AK
Principles & Interpretation of Laboratory Practices in Surgical Pathology (2016)
Hong-Wen Gao et al.
Amino acids, 36(2), 251-260 (2008-03-28)
We studied the non-specific interactions of two azo compounds: biebrich scarlet (BS) and naphthochrome green (NG), with four model proteins: bovine serum albumin, ovalbumin, poly-L-lysine and hemoglobin by UV-VIS spectrometry, fluorophotometry and circular dichroism melting technique. The optimal acidities of
Development of a bone reconstruction technique using a solid free-form fabrication (SFF)-based drug releasing scaffold and adipose-derived stem cells.
Lee JW, et al.
Journal of Biomedical Materials Research Part A, 101, 1865-1865 (2013)
Rob R Eversole et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(2), 253-257 (2003-01-21)
Here we describe a simple histochemical technique that provides an improved approach to identifying eosinophil components in tissues through the formation of photoreactive complexes that produce stable fluorescent emissions. This method worked readily with histological tissue sections 6-60 microm thick
View All Related Papers

Global Trade Item Number

SKUGTIN
B6008-100G04061833434390
B6008-25G04061835345878

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