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E6511

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate

Name: Ethylenediaminetetraacetic acid tetrasodium salt dihydrate
Brand: SIGMA

BioReagent, suitable for cell culture, 98.5-102.0%

Synonym(s):

EDTA tetrasodium salt, Edathamil, Tetrasodium ethylenediaminetetraacetate dihydrate

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About This Item

Linear Formula:

(NaOOCCH₂)₂NCH₂CH₂N(CH₂COONa)₂ · 2H₂O

CAS Number:

10378-23-1

Molecular Weight:

416.20

UNSPSC Code:

12352204

NACRES:

NA.75

PubChem Substance ID:

24894629

EC Number:

200-573-9

Beilstein/REAXYS Number:

3861753

MDL number:

MFCD00150026

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product line

BioReagent

Quality Level

200

assay

98.5-102.0%

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H₂O: 160 mg/mL

shipped in

ambient

storage temp.

room temp

SMILES string

O.O.[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.4Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;;2*1H2/q;4*+1;;/p-4

InChI key

KSYNLCYTMRMCGG-UHFFFAOYSA-J

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Related Categories

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Cell Dissociation Reagents

Molecular Biology & Functional Genomics

General description

Ethylenediaminetetraacetic acid (EDTA) is a chelator of metal ions. It is a substituted diamine, which has antibacterial activity. EDTA removes the undesirable effects of ferric, cupric and manganic ions in bleaching. It prevents cellular division, chlorophyll synthesis and algal biomass production. EDTA is an inhibitor of metalloprotease. It has anticoagulant property.

Application

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate has been used:

as a chelating agent to isolate human endometrial stem cell/stromal cells (hEnSCs) from menstrual blood
as a chelator in animal and testicular cell isolation
as a chelator for the culture of neural stem cells

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is used to eliminate enzyme inhibition by traces of heavy metals, and to inhibit enzymes that require divalent cations as cofactors.

pictograms

GHS08,GHS05,GHS07

signalword

Danger

hcodes

H302 + H332,H318,H373

pcodes

P260 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

0000548963

BCCP4651

0000518754

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What is the best protocol to cryopreserve immature mouse testicular cell suspensions?

Onofre J, et al.

Reproductive Biomedicine Online, 37(1), 6-17 (2018)

Modulation of matrix metalloproteinase activity by EDTA prevents posterior capsular opacification

Hazra S, et al.

Molecular Vision, 18(6), 1701-1701 (2012)

Human Endometrial Stem Cell Isolation from Endometrium and Menstrual Blood

Tavakol S, et al.

Bio-protocol, 8(2) (2018)

An optimized protocol for in vitro and in cellulo structural determination of the multi-tRNA synthetase complex by cross-linking mass spectrometry.

Krishnendu Khan et al.

STAR protocols, 3(1), 101201-101201 (2022-03-15)

Despite recent advances in structural determination of individual proteins, elucidating the 3-dimensional architecture of large, multiprotein complexes remains challenging, partly because of issues related to structural integrity during purification. Here, we describe a protocol to determine the 3-dimensional architecture of

Effect on cell surface markers following allergen-induced desensitization of human whole-blood basophils.

Gitte Lund et al.

International archives of allergy and immunology, 153(4), 323-334 (2010-06-19)

Allergen-specific immunotherapy (SIT) leads to reduced symptoms upon allergen exposure through as yet unresolved mechanisms. Desensitization of basophils to specific allergens during the updosing phase of injection immunotherapy may contribute to the clinical effect of SIT. Here we report a

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Global Trade Item Number

SKU	GTIN
E6511-100G	04061833604564
E6511-500G	04061833604571

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