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E9030

Endoglycoceramidase II from Rhodococcus sp.

Name: Endoglycoceramidase II from <I>Rhodococcus</I> sp.
Brand: SIGMA

aqueous solution

Synonym(s):

EGCase, ceramide glycanase, glycosyl-N-acetyl-sphingosine 1,1-β-D-glucanohydrolase, oligoglycosylglucosyl(1↔1)ceramide glycohydrolase, oligoglycosylglucosylceramide glycohydrolase

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.2.1.123 (BRENDA, IUBMB)

MDL number:

MFCD03456712

Recombinant:

expressed in E. coli

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Properties

recombinant

expressed in E. coli

Quality Level

200

conjugate

(Lipid-linked)

form

solution

mol wt

58.9 kDa

shipped in

dry ice

storage temp.

−20°C

Description

Application

Endoglycoceramidase II from Rhodococcus sp. has been used in a study to assess the differentiation of glycosphingolipid-derived glycan structural isomers by liquid chromatography and mass spectrometry. Endoglycoceramidase II from Rhodococcus sp. has also been used in a study to investigate structural and mechanistic analyses of endo-glycoceramidase II.

Physical form

Solution in 20 mM sodium acetate buffer, pH 6.0, containing 0.2% BSA and 0.1% Lubrol PX.

Other Notes

One unit will hydrolyze 1 μmol of asialo-GM1 per min at 37 °C at pH 5.0.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Novel fucogangliosides found in human colon adenocarcinoma tissues by means of glycomic analysis.

Hiroaki Korekane et al.

Analytical biochemistry, 364(1), 37-50 (2007-03-14)

The structures of acidic glycosphingolipids in colon adenocarcinoma have been analyzed extensively using a number of conventional methods, such as thin-layer chromatography and methylation analysis, and a variety of acidic glycosphingolipids present in the tissues have been reported. However, because

Biologically active glycosides from asteroidea, 42. Isolation and structure of a new biologically active ganglioside molecular species from the starfish Asterina pectinifera.

Ryuichi Higuchi et al.

Chemical & pharmaceutical bulletin, 54(3), 287-291 (2006-03-02)

A ganglioside molecular species GP-3 (1) has been obtained from the water-soluble lipid fraction of the chloroform/methanol extract of the starfish Asterina pectinifera. The structure of the ganglioside has been determined on the basis of chemical and spectroscopic evidence. Compound

Structural and mechanistic analyses of endo-glycoceramidase II, a membrane-associated family 5 glycosidase in the Apo and GM3 ganglioside-bound forms.

Matthew E C Caines et al.

The Journal of biological chemistry, 282(19), 14300-14308 (2007-03-03)

endo-Glycoceramidase, a membrane-associated family 5 glycosidase, deviates from the typical polysaccharide substrate specificity of other soluble members of the family, preferentially hydrolyzing glycosidic linkages between the oligosaccharide and ceramide moieties of gangliosides. Here we report the first x-ray crystal structures

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Global Trade Item Number

SKU	GTIN
E9030-100MUN	04061832843483