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F1141

Bovine Fibronectin

Name: Bovine Fibronectin
Brand: SIGMA

from bovine plasma, liquid, suitable for cell culture

Synonym(s):

Fibronectin

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About This Item

UNSPSC Code:

12352202

eCl@ss:

42020141

NACRES:

NA.75

EC Number:

289-149-2

MDL number:

MFCD00131062

Biological source:

bovine plasma

Form:

solution

Technique(s):

cell culture | mammalian: suitable

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Properties

Product Name

Fibronectin bovine plasma, solution, sterile-filtered, BioReagent, suitable for cell culture

biological source

bovine plasma

Quality Level

200

sterility

sterile-filtered

product line

BioReagent

form

solution

packaging

pkg of 1 mg, pkg of 2 mg, pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm²

UniProt accession no.

P07589

binding specificity

Peptide Source: Collagen
, Peptide Source: Fibrinogen

shipped in

wet ice

storage temp.

2-8°C

Gene Information

bovine ... FN1(280794)

Description

General description

Fibronectin is a ubiquitous protein of the extracellular matrix. It is a mosaic protein, that is made up of three types of repeating modular consensus amino acid sequences. Fibronectins belongs to a class of high-molecular-weight glycoproteins. Cellular fibronectin is expressed by fibroblasts and plasma fibronectin is usually liberated by hepatocytes.

Application

Fibronectin (FN) bovine plasma has been used in:

fibronectin and adsorption analysis (ELISA (enzyme-linked immunosorbent assay))
transwell migration
fibronectin assay

Biochem/physiol Actions

Fibronectins participate in cell adhesion, cytoskeletal organization, oncogenic transformation, cell migration, phagocytosis, hemostasis, and embryonic differentiation.

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Other Notes

Product F1141, Fibronectin from bovine plasma is a solution in 0.05 M TBS pH 7.5.

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pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Information

监管及禁止进口产品

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Certificates of Analysis (COA)

Lot/Batch Number

0000518561

0000463185

0000463182

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Protocols

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

Articles

Attachment Factors for Three-dimensional Cell Culture

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

View All Articles

Role of subnano-, nano-and submicron-surface features on osteoblast differentiation of bone marrow mesenchymal stem cells

Khang D, et al.

Biomaterials, 33(26), 5997-6007 (2012)

CCL2/CCR2 chemokine signaling coordinates survival and motility of breast cancer cells through Smad3 and p42/44MAPK dependent mechanisms

Fang W B, et al.

The Journal of biological chemistry, jbc-M112 (2012)

Enhanced fibronectin adsorption on carbon nanotube/poly (carbonate) urethane: independent role of surface nano-roughness and associated surface energy