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Merck
CN

G9891

β-Glycerophosphate disodium salt hydrate

powder, BioReagent, suitable for cell culture

Synonym(s):

BGP, Glycerol 2-phosphate disodium salt hydrate

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About This Item

Linear Formula:
(HOCH2)2CHOP(O)(ONa)2 · xH2O
CAS Number:
Molecular Weight:
216.04 (anhydrous basis)
UNSPSC Code:
12352200
PubChem Substance ID:
EC Number:
212-464-3
MDL number:
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product line

BioReagent

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

≤0.20% α-isomer

mp

102-104 °C (lit.)

SMILES string

O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O

InChI

1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2

InChI key

ROPZSVKNEIIIDE-UHFFFAOYSA-L

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Application

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.
Glycero-2-phosphate is a component of dexamethasone based hormone cocktail used to induce mesechymal stem cell (MSC) differentiation into osteoblasts.
Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Natsumi Watanabe et al.
Human gene therapy, 24(3), 283-294 (2013-01-31)
Hemophilia is an X-linked bleeding disorder, and patients with hemophilia are deficient in a biologically active coagulation factor. This study was designed to combine the efficiency of lentiviral vector transduction techniques with murine adipose tissue-derived stem/stromal cells (mADSCs) as a
Wen-Ning Xu et al.
Frontiers in endocrinology, 10, 922-922 (2020-02-13)
Long-term and high dose glucocorticoid treatment can cause decreased viability and function of osteoblasts, which leads to osteoporosis and osteonecrosis. In this study, we investigated the role and mechanism of action of HIF-1α in glucocorticoid-induced osteogenic inhibition in MC3T3-E1 cells.
Mario-Luca Suvà et al.
Cancer research, 69(5), 1776-1781 (2009-02-12)
Cancer stem cells that display tumor-initiating properties have recently been identified in several distinct types of malignancies, holding promise for more effective therapeutic strategies. However, evidence of such cells in sarcomas, which include some of the most aggressive and therapy-resistant
Monica Echeverry-Rendon et al.
Colloids and surfaces. B, Biointerfaces, 193, 111153-111153 (2020-06-07)
Magnesium (Mg) is a material widely used in industrial applications due to its low weight, ductility, and excellent mechanical properties. For non-permanent implants, Mg is particularly well-suited because of its biodegradability, while its degradation products are not harmful. However, Mg
Nastaran Abbarin et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 30(5), 775-785 (2014-11-20)
Amelotin (AMTN) is a recently discovered protein that is specifically expressed during the maturation stage of dental enamel formation. It is localized at the interface between the enamel surface and the apical surface of ameloblasts. AMTN knock-out mice have hypomineralized

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