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Merck
CN

L3289

Lysis solution for blood

sufficient for 100 reactions, Molecular Biology

Synonym(s):

Blood direct PCR, Blood lysis buffer, Extract-N-Amp blood direct PCR lysis solution

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About This Item

NACRES:
NA.25
UNSPSC Code:
12352200
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grade

Molecular Biology

Quality Level

form

liquid

usage

sufficient for 100 reactions

storage temp.

−20°C

General description

Lysis solution for blood in combination with neutralization solution for blood (N9784) aids in rapid extraction and neutralization of DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

This lysis solution is a component of the Extract-N-Amp and the REDExtract-N-Amp Blood PCR Kits

Application

Lysis solution for blood has been used for lysis of blood cells for DNA extraction prior to polymerase chain reaction (PCR) amplification. It has also been used for the isolation of genomic DNA for CRISPR-Cas9 gene editing.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC


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pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Identification by random mutagenesis of functional domains in KREPB5 that differentially affect RNA editing between life cycle stages of Trypanosoma brucei
McDermott S M, et al.
Molecular and Cellular Biology, MCB-00790 (2015)
Leandro S Thiago et al.
Haematologica, 99(1), 155-162 (2013-07-23)
The B-cell compartment in which multiple myeloma stem cells reside remains unclear. We investigated the potential presence of mature, surface-membrane immunoglobulin-positive B lymphocytes clonally related to the tumor bone marrow plasma cells among different subsets of peripheral blood B cells
Identification by random mutagenesis of functional domains in KREPB5 that differentially affect RNA editing between life cycle stages of Trypanosoma brucei
McDermott S M, et al.
Molecular and cellular biology, MCB-00790 (2015)



Global Trade Item Number

SKUGTIN
L3289-2.5ML04061837692444
L3289-.3ML04061837299629