M2694
Anti-Munc-18-1 antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-Munc-18α, Anti-STXBP1, Anti-Syntaxin-binding protein 1, Anti-rb-Sec-1
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IF, WB
Citations:
6
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 67 kDa
species reactivity
mouse, rat
technique(s)
indirect immunofluorescence: 1:250-1:500 using mouse fibroblast NIH3T3 cell line, microarray: suitable, western blot: 1:2,000-1:4,000 using rat brain extract (S1 fraction) and PC12 whole cell extract.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... STXBP1(6812)
mouse ... Stxbp1(20910)
rat ... Stxbp1(25558)
General description
Munc-18-1 (67 kDa) is an abundant neuronal protein that tightly binds to the synaptic fusion protein syntaxin. It is primarily expressed in neuronal tissues. Both Munc-18-1 and 2 isoforms bind tightly to syntaxins 1A, 2, and 3.
Immunogen
synthetic peptide corresponding to amino acids 577-594 located at the C-terminus of rat Munc-18-1 conjugated to KLH. This sequence is identical in mouse, chicken, bovine, and human Munc-18-1. It does not share homology with the Munc-18-2 and Munc-18-3 isoforms.
Application
Anti-Munc-18-1 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
Munc-18-1 functions in synaptic vesicle exocytosis. It modulates neurotransmission by associating with syntaxin 1. Munc-18-1 binds to both syntaxin 1A and 1B isoforms, inhibiting the binding of t-soluble N-ethylmaleimide-sensitive factor-attached protein receptor (SNARE) synaptobrevin/vesicle-associated membrane protein (VAMP) and SNAP25 to syntaxin 1 and preventing SNARE assembly. Munc-18 is required for neurotransmitter secretion from synaptic vesicles throughout development and has been implicated in axonal branching in hippocampal neurons.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Related Content
Datasheet
Gülçin Vardar et al.
eLife, 10 (2021-08-25)
Syntaxin-1 (STX1) and Munc18-1 are two requisite components of synaptic vesicular release machinery, so much so synaptic transmission cannot proceed in their absence. They form a tight complex through two major binding modes: through STX1's N-peptide and through STX1's closed
Mints as adaptors Direct binding to neurexins and recruitment of munc18
Biederer T and Sudhof TC
Test, 275(51), 39803-39806 (2000)
Ouada Nebie et al.
Brain : a journal of neurology, 144(10), 3142-3158 (2021-06-05)
Traumatic brain injury (TBI) leads to major brain anatomopathological damages underlined by neuroinflammation, oxidative stress and progressive neurodegeneration, ultimately leading to motor and cognitive deterioration. The multiple pathological events resulting from TBI can be addressed not by a single therapeutic
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M2694-200UL | 04061837720703 |