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M4526

Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

Alpha Modification, with sodium bicarbonate and Earl's salts, without ʟ-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

αMEM, EMEM, MEM

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.71

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Properties

Product Name

Minimum Essential Medium Eagle, Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

Earle’s salts (5% CO₂): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO₂): no
phenol red: yes
NaHCO₃: yes
stable glutamine: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.

This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins.

Application

Minimum Essential Medium Eagle has been used to maintain:

immature cumulus-oocyte complexes from mice
human mesenchymal stem cells (MSC)
mouse pre-osteoblastic cell line

Preparation Note

Supplement with 0.292 g/L L-glutamine.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
M4526-500ML	04061834059004
M4526-6X500ML	04061834059011
M4526-24X500ML	04061834058991