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M8407

Methotrexate hydrate

powder, suitable for cell culture, BioReagent

Synonym(s):

4-Amino-10-methylfolic acid hydrate, L-4-Amino-N10-methylpteroylglutamic acid hydrate, L-Amethopterin hydrate, Antifolan hydrate, MTX hydrate, Methylaminopterin hydrate

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About This Item

Empirical Formula (Hill Notation):
C20H22N8O5 · xH2O
CAS Number:
133073-73-1
Molecular Weight:
454.44 (anhydrous basis)
UNSPSC Code:
12352207
PubChem Substance ID:
24897252
NACRES:
NA.75
MDL number:
MFCD00150847
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Product Name

Methotrexate hydrate, powder, BioReagent, suitable for cell culture, ≥98% (HPLC)

product line

BioReagent

Quality Level

200

assay

≥98% (HPLC)

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: insoluble

antibiotic activity spectrum

neoplastics, parasites

mode of action

enzyme | inhibits

storage temp.

−20°C

SMILES string

[H]O[H].CN(Cc1cnc2nc(N)nc(N)c2n1)c3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O

InChI

1S/C20H22N8O5.H2O/c1-28(9-11-8-23-17-15(24-11)16(21)26-20(22)27-17)12-4-2-10(3-5-12)18(31)25-13(19(32)33)6-7-14(29)30;/h2-5,8,13H,6-7,9H2,1H3,(H,25,31)(H,29,30)(H,32,33)(H4,21,22,23,26,27);1H2/t13-;/m0./s1

InChI key

FPJYMUQSRFJSEW-ZOWNYOTGSA-N

Gene Information

human ... DHFR(1719)

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Application

Methotrexate hydrate has been used:
  • as a dihydrofolate (DHFR) inhibitor in sphere forming assay culture
  • as a component of dulbecco′ modified Eagle′s medium (DMEM) for selection of transfected cells
  • in viability and co-culture assays

Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems.
Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.
Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.

Biochem/physiol Actions

Methotrexate is a folic acid antagonist.
Methotrexate used to inhibit dihyrofolate reductase and select for DHFR overexpresssion.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
BCCB6273
BCCL7001
BCCJ7240
BCCG2425
BCCF4867

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Folate supplementation during methotrexate therapy for patients with psoriasis
Strober BE and Menon K
Journal of the American Academy of Dermatology, 53(4), 652-659 (2005)
Localizations of Na+-d-glucose cotransporters SGLT1 and SGLT2 in human kidney and of SGLT1 in human small intestine, liver, lung, and heart
Vrhovac I, et al.
Pflugers Archiv : European Journal of Physiology, 467(9), 1881-1898 (2015)
Comparative metabolic analysis of CHO cell clones obtained through cell engineering, for IgG productivity, growth and cell longevity
Wilkens CA and Gerdtzen ZP
Testing, 10(3), e0119053-e0119053 (2015)
Methylthioadenosine (MTA) Rescues Methylthioadenosine Phosphorylase (MTAP)-deficient tumors from purine synthesis inhibition in vivo via non-autonomous adenine supply
Ruefli-Brasse A, et al.
Journal of Cancer Therapy, 2(4), 523-523 (2011)
Cross talk between one-carbon metabolism, Eph signaling, and histone methylation promotes neural stem cell differentiation
Fawal MA, et al.
Testing, 23(10), 2864-2873 (2018)
View All Related Papers

Global Trade Item Number

SKUGTIN
M8407-500MG04061834063063
M8407-100MG04061835568956

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