M8883
4-Methylumbelliferyl phosphate
phosphatase substrate, fluorogenic, ≥98% (HPLC), powder
Synonym(s):
4-Methylumbelliferyl-phosphoric acid
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About This Item
Empirical Formula (Hill Notation):
C10H9O6P
CAS Number:
Molecular Weight:
256.15
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
222-137-7
MDL number:
Beilstein/REAXYS Number:
1687229
Product Name
4-Methylumbelliferyl phosphate, phosphatase substrate
Quality Level
assay
≥98% (HPLC)
form
powder
solubility
water: 25 mg/mL, clear to very slightly hazy, colorless
fluorescence
λex 319 nm; λem 384 nm (pH 9.1), λex 360 nm; λem 449 nm (Reaction product)
storage temp.
−20°C
SMILES string
CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12
InChI
1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
InChI key
BCHIXGBGRHLSBE-UHFFFAOYSA-N
General description
Fluorogenic substrate for phosphatases.
Application
4-Methylumbelliferyl phosphate has been used as a substrate for alkaline phosphatase in the peptide-binding assay. It has also been used as a fluorogenic substrate in enzyme-linked immunosorbent assay (ELISA).
Biochem/physiol Actions
4-Methylumbelliferyl phosphate serves as a fluorogenic substrate for calmodulin-dependent protein phosphatase and in kinetic studies of alkaline phosphatase. It is a substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. 4-Methylumbelliferyl phosphate is seven times more sensitive than phenolphthalein monophosphate and 8-13 times more sensitive than with p-nitrophenyl phosphate when used in enzyme immunoassays for the detection of antibodies to human immunodeficiency viruses.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Yuka Hama et al.
Nutrients, 12(10) (2020-10-18)
Although folate deficiency was reported to be associated with hyperhomocysteinemia, influence of folate supplementation on cognition remains controversial. Therefore, we explored the effects of folate supplementation on the cognition and Homocysteine (Hcy) level in relatively short periods in patients with
Francesca Bertolini et al.
Journal of pharmaceutical sciences, 96(11), 2931-2944 (2007-08-21)
Oxidative damage to proteins, implicated amongst other in the etiology and progression of Parkinson's disease (PD) and Alzheimer's disease (AD), results in the loss of specific biological protein function. A simple, sensitive, and cost-effective fluorimetric test to assess the antioxidant
Laurent Gilardin et al.
Haematologica, 102(11), 1833-1841 (2017-07-29)
Acquired thrombotic thrombocytopenic purpura is a rare and severe disease characterized by auto-antibodies directed against "A Disintegrin And Metalloproteinase with Thrombospondin type 1 repeats, 13
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M8883-250MG | 04061832909363 |
| M8883-5G | 04061835512751 |
| M8883-100MG | 04061835557752 |
| M8883-1G | 04061834063674 |