Skip to Content
Merck
CN

M9015

Malachite Green oxalate salt

Dye content, ≥90%, certified by the Biological Stain Commission, powder or crystals

Synonym(s):

N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbenium oxalate, Basic Green 4

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C23H25N2 · C2HO4 · 0.5C2H2O4
CAS Number:
2437-29-8
Molecular Weight:
463.50
NACRES:
NA.47
PubChem Substance ID:
24897308
UNSPSC Code:
12171500
EC Number:
219-441-7
Beilstein/REAXYS Number:
3644550
Colour Index Number:
42000

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Malachite Green oxalate salt, certified by the BSC

agency

certified by the BSC

Quality Level

200

form

powder or crystals

composition

Dye content, ≥90%

technique(s)

microbe id | staining: suitable

color

faint green to very dark green

mp

144-150 °C

solubility

H2O: 1 mg/mL, blue to very deep blue

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

C[N+](C)=C1C=CC(C=C1)=C(C2=CC=C(N(C)C)C=C2)C3=CC=CC=C3.[O-]C(C(O)=O)=O

InChI

1S/C23H25N2.C2H2O4/c1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3-1(4)2(5)6/h5-17H,1-4H3;(H,3,4)(H,5,6)/q+1;/p-1

InChI key

REZFCYOVFCJTDG-UHFFFAOYSA-M

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Malachite Green is a basic dye belonging to the triphenylmethane group. It is required in biology for the staining of microscopic samples, such as plant tissue, yeast and bacteria. The malachite green method is a sensitive method for the detection of released phosphate.

Application

Malachite Green oxalate salt has been used for phosphate quantification.
Malachite Green oxalate salt has been used:
  • in the preparation of Alexander staining solution for cell viability test
  • in malachite green-ammonium molybdate assay for determining the adenosine triphosphatase (ATPase) activity of binding immunoglobulin protein (BiP)
  • as a hydrophobic ion, to study the label-free molecular transport in the living cell membranes of Escherichia coli in real time by second-harmonic generation (SHG) method

Certified for use as a counterstain for paraffin embedded botanical material; as a spore stain for bacteria by the method of Schaeffer and Fulton.

Other Notes

The molecular mass of malachite green oxalate is expressed as either:
927.00 g/mol - the mass of 2 mol malachite green + 2 mol monohydrogen oxalate + 1 mol dihydrogen oxalate, or
463.50 g/mol - the mass of 1 mol malachite green + 1 mol monohydrogen oxalate + 0.5 mol dihydrogen oxalate

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SHBT2592
SHBR9077
SHBS2183
SHBQ8468
SHBQ1550

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Biophysical analysis of the effect of chemical modification by 4-oxononenal on the structure, stability, and function of binding immunoglobulin protein (BiP)
Shah DD, et al.
Testing, 12(9), e0183975-e0183975 (2017)
Synthetic Dyes in Biology, Medicine And Chemistry (2012)
AUTEN-67, an autophagy-enhancing drug candidate with potent antiaging and neuroprotective effects.
Papp D, et. al.
Autophagy, 12, 273-273 (2016)
Methods in Plant Cell Biology (1995)
Rapid separation of Arabidopsis male gametophyte developmental stages using a Percoll gradient
Duplakova N, et al.
Nature Protocols, 11(10), 1817-1817 (2016)
View All Related Papers

Global Trade Item Number

SKUGTIN
M9015-25G04061834063858
M9015-100G04061834063841

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service