Mitochondria Isolation Kit

Name: Mitochondria Isolation Kit
Brand: SIGMA

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

Synonym(s):

Mitochondria Purification Kit

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About This Item

UNSPSC Code:

12352200

EC Number:

232-650-8

NACRES:

NA.32

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Properties

Quality Level

200

usage

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL)

shelf life

2 yr

technique(s)

fractionation: suitable

shipped in

dry ice

storage temp.

−20°C

Description

Application

Mitochondria Isolation Kit has been used:

for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity
for the isolation of mitochondria from glomeruli and heart
for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential

The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.

Features and Benefits

Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane