N2770
p-Nitrophenyl phosphate
SIGMAFAST™, chromogenic, tablet
Synonym(s):
pNPP tablets
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About This Item
Product Name
SIGMAFAST™ p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL
Quality Level
form
tablet
solubility
water: 0.25 tablet/mL, clear, colorless
storage temp.
−20°C
Application
Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each SIGMAFAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.
Preparation Note
Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing pNPP.
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| N2770-50SET | 04061835557998 |
| N2770-5SET | 04061834110491 |