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NXTRACT

NuCLEAR™ Extraction Kit

For mammalian tissue or cultured cells

Synonym(s):

Nuclear Isolation Kit

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About This Item

NACRES:
NA.56
UNSPSC Code:
41105517
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Quality Level

300

usage

 kit sufficient for 10 extractions (1 ml packed cell volume),  kit sufficient for 100 extractions (100 μl packed cell volume)

technique(s)

protein extraction: suitable, western blot: suitable

shipped in

dry ice

storage temp.

−20°C

General description

The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.

Application

NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice. It was also used to test the therapeutic potential of andrographolide for treating endometriosis.

Other Notes

A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. NuCLEAR offers the flexiblity you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts.
Recommended Antibodies for Immunodetection L1293, N2662, AMAB90549
Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.

Legal Information

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

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Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • 3× Dilution and Equilibration Buffer 90 mL
    SDS
  • P8340
    Protease Inhibitor Cocktail 1 mL
    SDS

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

wgk

WGK 3

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Regulatory Information

常规特殊物品
低风险生物材料

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000536295
0000149724
0000149724
0000114896
059M4108V

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Articles

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

细胞器分离

亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。

F Guidez et al.
Molecular and cellular biology, 18(7), 3851-3861 (1998-06-25)
Granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF) independently stimulate the proliferation and differentiation of macrophages from bone marrow progenitor cells. Although the GM-CSF and M-CSF receptors are unrelated, both couple to Ras-dependent signal transduction pathways, suggesting that these
Qing Wang et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 302(9), R1025-R1033 (2012-03-10)
We previously reported that excess of deoxycorticosterone-acetate (DOCA)/salt-induced cardiac hypertrophy in the absence of hypertension in one-renin gene mice. This model allows us to study molecular mechanisms of high-salt intake in the development of cardiovascular remodeling, independently of blood pressure
K A Lee et al.
Gene analysis techniques, 5(2), 22-31 (1988-03-01)
A convenient and rapid method for preparing soluble extracts from the nuclei of as few as 3 x 10(7) mammalian cells (miniextract procedure) is described. By several criteria, miniextracts are comparable to nuclear extracts prepared from large numbers of cells
View All Related Papers

Global Trade Item Number

SKUGTIN
NXTRACT-1KT04061838256133