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T3073

Tissue Preparation Solution

Name: Tissue Preparation Solution
Brand: SIGMA

30 mL sufficient for 1,000 extractions

Synonym(s):

Direct PCR tissue preparation solution, Extract-N-Amp^™ Tissue Preparation Solution, REDExtract-N-Amp^™ Tissue Preparation Solution, SYBR^® Green Extract-N-Amp^™ Tissue Preparation Solution

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About This Item

UNSPSC Code:

12352200

EC Number:

254-457-8

NACRES:

NA.55

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Properties

Quality Level

200

usage

30 mL sufficient for 1,000 extractions, sufficient for 1000 reactions

feature

Direct PCR

technique(s)

PCR: suitable

input

crude DNA

shipped in

wet ice

storage temp.

−20°C

Description

General description

The Tissue Preparation Solution is intended for use with the Extract-N-Amp^™ Tissue PCR kits, REDExtract-N-Amp^™ Tissue PCR kits, and SYBR^® Green Extract-N-Amp Tissue PCR kits. This product is intended to supplement the kit reagents, where there are many extractions.

Application

Tissue preparation solution has been used:

in combining segments of tails for genotyping
to extract genomic DNA from zebrafish embryos/fin tissues
to extract DNA from ear tags, tail tips and embryonic yolk sacs of mouse embryo
for extraction of DNA from fungal spores and mycelia

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Protocols

REDExtract-N-Amp™ Tissue PCR Kit Protocol

Extract-N-Amp kit protocol provides rapid DNA extraction yielding PCR-ready samples in 15 minutes with optimized PCR ReadyMix.

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

REDExtract-N-Amp™组织PCR试剂盒实验方案

exrract-N-Amp试剂盒实验方案提供了一种快速的DNA提取方法，可在15分钟内获得PCR样品。优化的PCR ReadyMix能成功产生清洁的扩增。RED上样染料实现样品跟踪。

In vitro experimental environments lacking or containing soil disparately affect competition experiments of Aspergillus flavus and co-occurring fungi in maize grains.

Titilayo D O Falade et al.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 33(7), 1241-1253 (2016-06-07)

In vitro experimental environments are used to study interactions between microorganisms, and to predict dynamics in natural ecosystems. This study highlights that experimental in vitro environments should be selected to match closely the natural environment of interest during in vitro

N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment.

Soh BS

Cell Research, 24(12), 1420-1432 (2014)

A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish

Varshney GK, et al.

Nature Protocols, 11(12), 2357-2357 (2016)

View All Related Papers

Global Trade Item Number

SKU	GTIN
T3073-.3ML	04061838250131
T3073-3ML	04061838101266
T3073-30ML	04061833768112