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T3405

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

Name: 3,3&#8242;,5,5&#8242;-Tetramethylbenzidine dihydrochloride
Brand: SIGMA

chromogenic, tablet

Synonym(s):

4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride

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About This Item

Empirical Formula (Hill Notation):

C₁₆H₂₀N₂ · 2HCl

CAS Number:

64285-73-0

Molecular Weight:

313.27

NACRES:

NA.83

PubChem Substance ID:

24900140

UNSPSC Code:

12352204

EC Number:

264-769-6

MDL number:

MFCD00012961

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Properties

Product Name

3,3′,5,5′-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet

Quality Level

200

form

tablet

mp

≥300 °C

storage temp.

2-8°C

SMILES string

Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H

InChI key

NYNRGZULARUZCC-UHFFFAOYSA-N

Description

Application

3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the

anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Global Trade Item Number

SKU	GTIN
T3405-50TAB	04061837344404
T3405-5TAB	04061837344411
T3405-100TAB	04061835522309