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U5378

Urea

powder, BioReagent, Molecular Biology, suitable for cell culture

Synonym(s):

Carbamide, Carbonyldiamide

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About This Item

Linear Formula:
NH2CONH2
CAS Number:
57-13-6
Molecular Weight:
60.06
UNSPSC Code:
12352111
NACRES:
NA.31
PubChem Substance ID:
24900656
EC Number:
200-315-5
Beilstein/REAXYS Number:
635724
MDL number:
MFCD00008022
Assay:
≥98%
Grade:
Molecular Biology
Technique(s):
cell culture | mammalian: suitable

Key Documents

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grade

Molecular Biology

Quality Level

200

product line

BioReagent

assay

≥98%

form

powder

technique(s)

cell culture | mammalian: suitable

mp

132-135 °C (lit.)

solubility

H2O: soluble 480 mg/mL, clear, colorless (8M)

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 ppm

cation traces

Cu: ≤1 ppm, Fe: ≤5 ppm, Pb: ≤2 ppm

absorption

≤0.06 at 260 at 6 M, ≤0.06 at 280 at 6 M

foreign activity

DNase, NICKase, RNase and protease, none detected

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

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Application

  • Urea has been used in protein resuspension buffer and to denature proteins.
  • It has been used for the isolation of proteins via urea-containing polyacrylamide gels.
  • It has been used to study the conformation of unfolded proteins under different concentrations of urea and different pH.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF8492V
0000464164
BCCN1361
WXBF5575V
0000414069

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Labeling and identification of direct kinase substrates.
Carlson SM and White FM
Science Signaling, 5, pl3-pl3 (2012)
Construction and transfection of a ribozyme targeting human caspase-3.
Cronin D et al.
Anticancer Research, 24, 425-425 (2004)
Structure and properties of native and unfolded lysing enzyme from T. harzianum: Chemical and pH denaturation.
Bey H et al.
International Journal of Biological Macromolecules, 92, 860-860 (2016)
Ziyi Yin et al.
Autophagy, 15(7), 1234-1257 (2019-02-20)
Macroautophagy/autophagy is critical for normal appressorium formation and pathogenicity of the rice blast fungus Magnaporthe oryzae, but the molecular base of autophagy linked to pathogenicity remains elusive in this or other pathogenic fungi. We found that MoHat1, a histone acetyltransferase
Florin Sasarman et al.
Human molecular genetics, 24(10), 2841-2847 (2015-02-06)
Addition of the trinucleotide cytosine/cytosine/adenine (CCA) to the 3' end of transfer RNAs (tRNAs) is essential for translation and is catalyzed by the enzyme TRNT1 (tRNA nucleotidyl transferase), which functions in both the cytoplasm and mitochondria. Exome sequencing revealed TRNT1
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Product Information Sheet - U5378

Product Information Sheet

Global Trade Item Number

SKUGTIN
U5378-500G04061835550449
U5378-5KG04061835550456
U5378-1KG04061835550432
U5378-100G04061835522675

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