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Merck
CN

A2856

Anti-ATG7 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

ATG7 Antibody - Anti-ATG7 antibody produced in rabbit, Atg7 Antibody, Anti-APG7, Anti-APG7-like, Anti-APG7L, Anti-Apoptosis-specific-protein, Anti-Autophagy protein 7-like, Anti-Ubiquitin activating enzyme E1-like protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD09753848

Key Documents

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Product Name

Anti-ATG7 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~75 kDa

species reactivity

rat, human, mouse

packaging

antibody small pack of 25 μL

technique(s)

immunoprecipitation (IP): 1-2 μL using human U87 cell lysate
western blot: 0.5-1 μg/mL using whole extracts of mouse 3T3 cells

UniProt accession no.

O95352

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... ATG7(10533)
mouse ... Atg7(74244)
rat ... Atg7(312647)

Related Categories

Immunogen

synthetic peptide corresponding to amino acids 38-50 of human ATG7, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse and rat.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-ATG7 antibody can be used in western blotting and immunoblotting.
Anti-ATG7 antibody produced in rabbit has been used:
  • for quantitative real-time polymerase chain reaction of autophagy-related genes
  • in immunofluorescence and immunoblotting
  • in in vivo acetylation/deacetylation of autophagy proteins

Rabbit anti-ATG7 antibody has been used for immunoblotting assays. The antibody is also suitable for use in immunoprecipitation applications at 1-2μL using human U87 cell lysates.
Rabbit anti-ATG7 antibody was used for immunohistochemistry on sections of mouse midbrain fixed in 4% paraformaldehyde at 1:2000.

Biochem/physiol Actions

Autophagy related 7 (ATG7) regulates caspase mediated autophagy and apoptosis. This has a significant effect on tumor progression and ATG7 acts as a tumor suppressor. ATG7 is known to be associated with many cancer types, hence it is considered as a target for cancer therapy. Loss of ATG7 gene contributes to liver carcinogenesis. The ATG7 gene encodes E1-like enzyme essential for lipidation and conjugation systems : ubiquitin-like microtubule-associated protein 1A/1B-light chain 3 (LC3) and autophagy related 12 (ATG12) respectively.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Autophagy is a catabolic process by which cell delivers cytoplasmic constituent into lysosome for protein degradation. 16 ATG genes are essential for autophagosome preparation out of which Atg7 is the one required to activate ubiquitin-like proteins Atg12 and Atg8. Atg7 is also crucial for amino acid supply in neonates, as well as for bulk degradation of proteins and organelles in mice during starvation. Anti-ATG7 antibody can be used in immunohistochemistry and immunoprecipitation. Rabbit anti-ATG7 antibody reacts specifically with Atg7 of human, mouse and rat.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000488146
0000488146
0000466582
0000466582
0000386019

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Shinichi Kawano et al.
Biology open, 6(11), 1644-1653 (2017-10-04)
Adhesion of cells to the extracellular matrix (ECM) via focal adhesions (FAs) is crucial for cell survival, migration, and differentiation. Although the regulation of FAs, including by integrins and the ECM, is important to cell behavior, how FAs are regulated
Xiaoqi Xie et al.
Cancer discovery, 5(4), 410-423 (2015-02-13)
Macroautophagy (autophagy hereafter) may promote survival and growth of spontaneous tumors, including melanoma. We utilized a genetically engineered mouse model of melanoma driven by oncogenic BrafV600E and deficiency in the Pten tumor suppressor gene in melanocytes to test the functional
Anne M Strohecker et al.
Cancer discovery, 3(11), 1272-1285 (2013-08-24)
Autophagic elimination of defective mitochondria suppresses oxidative stress and preserves mitochondrial function. Here, the essential autophagy gene Atg7 was deleted in a mouse model of BrafV600E-induced lung cancer in the presence or absence of the tumor suppressor Trp53. Atg7 deletion
PSMD10/gankyrin induces autophagy to promote tumor progression through cytoplasmic interaction with ATG7 and nuclear transactivation of ATG7 expression
Luo T, et al.
Autophagy, 12(8), 1355-1371 (2016)
Man J Livingston et al.
Autophagy, 12(6), 976-998 (2016-04-29)
Renal fibrosis is the final, common pathway of end-stage renal disease. Whether and how autophagy contributes to renal fibrosis remains unclear. Here we first detected persistent autophagy in kidney proximal tubules in the renal fibrosis model of unilateral ureteral obstruction
View All Related Papers

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Data Sheet - A2856

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