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A4480

Anti-ASPP2 antibody, Mouse monoclonal

Name: Anti-ASPP2 antibody, Mouse monoclonal
Brand: SIGMA

~1.5 mg/mL, clone DX 54.10, purified from hybridoma cell culture

Synonym(s):

Anti-53BP2 apoptosis-stimulating protein of p53 2, Anti-TP53BP2, Anti-tumor protein p53-binding protein 2

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD08705575

Technique(s):

immunoprecipitation (IP): suitable, immunocytochemistry: suitable, microarray: suitable, western blot: 0.5-1 μg/mL using total cell extract of human osteogenic sarcoma

Conjugate:

unconjugated

Application:

ARR, ICC, IP, WB

Citations:

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

DX 54.10, monoclonal

form

buffered aqueous solution

mol wt

antigen ~175 kDa

species reactivity

human, mouse

concentration

~1.5 mg/mL

technique(s)

immunoprecipitation (IP): suitable, immunocytochemistry: suitable, microarray: suitable, western blot: 0.5-1 μg/mL using total cell extract of human osteogenic sarcoma

isotype

IgG1

UniProt accession no.

Q13625

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TP53BP2(7159)
mouse ... Trp53bp2(209456)

Description

General description

Monoclonal Anti-ASPP2 (mouse IgG1 isotype) is derived from the hybridoma DX 54.10 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice. Apoptosis-stimulating protein of p53 (ASPP) family of proteins contains three members, ASPP1, ASPP2 and iASPP, which interact via their C-terminal region with p53 and modulate its activity.

Immunogen

fragment of human ASPP2 (amino acids 691-1128).

Application

Mouse Monoclonal Anti-ASPP2 has been used for immunofluorescence analysis. The antibody can also be used for immunocytochemistry, immunoprecipitation and microarray applications.

Western blot analysis of Hek293 or 3T3-L1 adipocyte cell lysates was performed using monoclonal mouse anti-ASPP2 as the primary.

Biochem/physiol Actions

ASPP2 is a tumor suppressor that activates p53. ASPP2 also regulates Par-3 which subsequently modulates the proliferation and polarity of neural progenitor cells during CNS development. Furthermore, ASPP2 interacts with active Ras to induce p53 mediated cell death. ASPP2 is known to function as a biomarker for invasive pituitary adenomas.

Apoptosis-stimulating protein of p53 (ASPP2) enhances the ability of p53 to induce apoptosis ASPP1 and ASPP2 stimulates the transactivation function of p63 and p73 on promoters of different genes such as BCL2 associated X, apoptosis regulator (Bax), p53-inducible gene 3 (PIG3) and p53 upregulated modulator of apoptosis (PUMA), but not mouse double minute 2 homolog (Mdm2) or p21^WAF-1/CIP1.

Monoclonal Anti-ASPP2 recognizes human and mouse ASPP2. The epitope recognized by the antibody resides within amino acids 691-1128 of human ASPP2.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c