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A5060

Sigma-Aldrich

Anti-Actin (20-33) antibody produced in rabbit

enhanced validation

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Anti-ACTA
MDL number:
MFCD01092261
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

wide range (animal), invertebrates

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using chicken or rat tissue sections
indirect immunofluorescence: 1:200 using cultured human fibroblasts
microarray: suitable
western blot: 1:250 using using rat brain or chicken muscle extracts

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTA1(58)
mouse ... Acta1(11459)
rat ... Acta1(29437)

Related Categories

General description

A mature actin filament consists of a right-handed double helix made of two F-actin protofilaments.
The antibody shows a broad reactivity among actin isoforms and across a range of organisms. It specifically stains typical stress fibers in cultured cells using an indirect immunofluorescent labeling technique and can be used as a probe of the N-terminal region of actin.
Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle).

Specificity

Anti-Actin recognizes an epitope located on the N-terminal region of actin. The antibody specifically labels actin in a wide variety of tissues and species using immunoblotting (band at 42kDa). It specifically stains typical stress fibers in cultured cells using indirect immunofluorescent staining and immunohistochemistry.

Immunogen

N-terminal actin peptide attached to a multiple antigen peptide (MAP) backbone.

Application

Homogenates from limb tissue of E13.5 mouse embryos were analyzed by western blot using rabbit anti-actin as a primary antibody at a dilution of 1:8000.
Rabbit Anti-Actin (20-33) antibody has been used for immunoblotting assays. The product can also be used for immunohistochemistry, indirect immunofluorescence and microarray analyses.

Biochem/physiol Actions

Actin maintains the cell shape and participates in cytokinesis. Actin provides cellular strength and promotes active movement of the cell. Actin protein is essential for the formation of mature platelets. Upregulation of ACTA1 (actin, α 1) gene is observed in orthotopic liver transplantation without any earlier evidence of autoimmune liver disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative.

Storage and Stability

For continuous use, store at 2-8 °C for a maximum of one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Regulatory Information

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An actin-based cytoskeleton in archaea.
Ettema T J G, et al.
Molecular Microbiology, 80(4), 1052-1061 (2011)
Cytoskeletal regulation of platelet formation: Coordination of F-actin and microtubules.
Poulter N S and Thomas S G
The International Journal of Biochemistry & Cell Biology, 66, 69-74 (2015)
Lamellipodin promotes actin assembly by clustering Ena/VASP proteins and tethering them to actin filaments.
Hansen S D, et al.
eLife, 4, e06585-e06585 (2015)
Jaclyn Nicole Le Grand et al.
Autophagy, 7(11), 1302-1307 (2011-08-25)
Atg8 is a yeast protein involved in the autophagic process and in particular in the elongation of autophagosomes. In mammals, several orthologs have been identified and are classed into two subfamilies: the LC3 subfamily and the GABARAP subfamily, referred to
Lack of Clinical Relevance of ANA and ASMA Positivity in Patients with Liver Transplantation without a History of Autoimmune Diseases.
Pellegrini L, et al.
BioMed Research International, 2017 (2017)
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