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B4679

BIS-TRIS propane

Name: BIS-TRIS propane
Brand: SIGMA

BioPerformance Certified, suitable for cell culture, ≥99.0%

Synonym(s):

1,3-Bis[tris(hydroxymethyl)methylamino]propane

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About This Item

Linear Formula:

CH₂[CH₂NHC(CH₂OH)₃]₂

CAS Number:

64431-96-5

Molecular Weight:

282.33

UNSPSC Code:

12161700

NACRES:

NA.25

PubChem Substance ID:

24891799

EC Number:

264-899-3

Beilstein/REAXYS Number:

1786109

MDL number:

MFCD00004689

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grade

BioPerformance Certified

Quality Level

400

assay

≥99.0%

form

crystals

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and bioburden, tested

useful pH range

6.3-9.5

pKa (25 °C)

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)

application(s)

diagnostic assay manufacturing

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

SMILES string

OCC(CO)(CO)NCCCNC(CO)(CO)CO

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

InChI key

HHKZCCWKTZRCCL-UHFFFAOYSA-N

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Related Categories

Biochemicals

Biological Buffers

High-Purity Biochemicals and Reagents

Chemistry & Biochemicals

Application

Neutralized chimeric DNA probe for the improvement of GC-rich RNA detection specificity on the nanowire field-effect transistor.: BIS-TRIS propane is used in the development of nanowire field-effect transistors for detecting GC-rich RNA sequences. The study demonstrates the buffer′s role in enhancing the specificity and sensitivity of these biosensors (Chou et al., 2019).

Elimination of ghost peaks by optimization of anion exchange chromatography method for determination of gamma-carboxyglutamic acid (Gla)-domainless impurity in recombinant activated clotting factor VII drug products.: BIS-TRIS propane is employed in optimizing chromatography methods to eliminate ghost peaks during the analysis of pharmaceutical impurities. The buffer′s effectiveness ensures accurate and reliable detection of impurities in drug formulations (Tahmasbi et al., 2019).

Other Notes

Easily compare specifications for BIS-TRIS propane products with the BIS-TRIS propane specification table.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Buffer effects on electric signals of light-excited bacteriorhodopsin.

R Tóth-Boconádi et al.

Biophysical journal, 78(6), 3170-3177 (2000-05-29)

Buffers change the electric signals of light-excited bacteriorhodopsin molecules in purple membrane if their concentration and the pH of the low-salt solution are properly selected. "Positive" buffers produce a positive component, and "negative" buffers a negative component in addition to

Conduction and block by organic cations in a K+-selective channel from sarcoplasmic reticulum incorporated into planar phospholipid bilayers.

R Coronado et al.

The Journal of general physiology, 79(4), 529-547 (1982-04-01)

A collection of organic cations has been used to probe the gross structural features of the ionic diffusion pathway in a K+-selective channel from sarcoplasmic reticulum (SR). Channels were incorporated into planar phospholipid bilayer membranes, and single-channel currents were measured

Purification and characterization of a secreted arginine-specific dibasic cleaving enzyme from EL-4 cells.

E Csuhai et al.

Biochemistry, 34(38), 12411-12419 (1995-09-26)

A secreted dibasic cleaving peptidase capable of converting dynorphins into Leu-enkephalin-Arg6 was purified from the medium of EL-4 mouse thymoma cells. The enzyme is a novel metalloendopeptidase with a neutral pH optimum (6.9) and a molecular weight of approximately 130

Electroosmotic flow-balanced isotachophoretic stacking with continuous electrokinetic injection for the concentration of anions in high conductivity samples.

Michael C Breadmore

Journal of chromatography. A, 1217(24), 3900-3906 (2010-05-11)

An EOF counter-balanced ITP boundary has been used to stack anions from high conductivity samples during continuous electrokinetic injection of the sample. In a polystyrenesulfonate/poly(diallyldimethylammonium chloride) polyelectrolyte coated capillary, the time at which the ITP boundary exited the capillary could

Bovine cardiac myosin subfragment 1. Transient kinetics of ATP hydrolysis.

S J Smith et al.

The Journal of biological chemistry, 259(15), 9365-9368 (1984-08-10)

The kinetics of binding and hydrolysis of ATP by bovine cardiac myosin subfragment 1 has been reinvestigated. More than 90% of the total fluorescence amplitude associated with ATP hydrolysis occurs with an apparent second-order rate constant of 8.1 X 10(5)

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Global Trade Item Number

SKU	GTIN
B4679-25G	04061833374337
B4679-500G	04061833433416
B4679-100G	04061833617106

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