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Merck
CN

B8781

Restriction Enzyme Buffer SB

10 ×

Synonym(s):

Restriction Endonuclease Buffer

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About This Item

NACRES:
NA.53
UNSPSC Code:
12352204
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InChI

1S/C58H77N15O9/c1-32(2)21-39(29-65-46(52(61)76)22-35-13-7-6-8-14-35)69-57(81)48(25-38-28-62-31-67-38)70-50(75)30-66-58(82)51(33(3)4)73-53(77)34(5)68-56(80)47(24-37-27-64-44-18-12-10-16-41(37)44)72-55(79)45(19-20-49(60)74)71-54(78)42(59)23-36-26-63-43-17-11-9-15-40(36)43/h6-18,26-28,31-34,39,42,45-48,51,63-65H,19-25,29-30,59H2,1-5H3,(H2,60,74)(H2,61,76)(H,62,67)(H,66,82)(H,68,80)(H,69,81)(H,70,75)(H,71,78)(H,72,79)(H,73,77)

InChI key

YNZJHLFHFNCZIE-UHFFFAOYSA-N

SMILES string

CC(C)CC(CNC(Cc1ccccc1)C(N)=O)NC(=O)C(Cc2cnc[nH]2)NC(=O)CNC(=O)C(NC(=O)C(C)NC(=O)C(Cc3c[nH]c4ccccc34)NC(=O)C(CCC(N)=O)NC(=O)C(N)Cc5c[nH]c6ccccc56)C(C)C

grade

Molecular Biology

description

10× buffer contains 100 mM Tris-HCl, pH 8.0, 50 mM MgCl2, 1000 mM NaCl, 10 mM 2-mercaptoethanol at 37 °C.

form

buffered aqueous solution

concentration

10 ×

storage temp.

−20°C

Quality Level

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General description

This buffer is for use with Sigma Restriction Enzymes. Please note every restriction enzyme is supplied with the appropriate buffer - this product is available for users who need additional buffer. For details on buffer composition and protocols for use, see the "Protocols and Articles" tab above.

Application

Restriction Enzyme Buffer SB has been used in restriction digestion of PCR amplified product.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+ C nucleotide content and secondary structure melting point
Bukovska P, et al.
Journal of Microbiological Methods, 82(3), 223-228 (2010)

Articles

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

术语“限制酶”源自Werner Arber和Matthew Meselson实验室对肠杆菌噬菌体λ(λ噬菌体)的研究。

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