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Merck
CN

C2278

CAPSO

≥99% anhydrous basis (titration)

Synonym(s):

3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid, CAPSO Free Acid

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About This Item

Empirical Formula (Hill Notation):
C9H19NO4S
CAS Number:
73463-39-5
Molecular Weight:
237.32
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24892483
MDL number:
MFCD00041778
Beilstein/REAXYS Number:
5939234

Key Documents

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Product Name

CAPSO, ≥99% anhydrous basis (titration)

InChI key

INEWUCPYEUEQTN-UHFFFAOYSA-N

SMILES string

OC(CNC1CCCCC1)CS(O)(=O)=O

InChI

1S/C9H19NO4S/c11-9(7-15(12,13)14)6-10-8-4-2-1-3-5-8/h8-11H,1-7H2,(H,12,13,14)

assay

≥99% anhydrous basis (titration)

form

crystalline powder

pH

2.5-4.0

useful pH range

8.9-10.3

pKa (25 °C)

9.6

solubility

water: 0.111 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

Quality Level

200

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Application

CAPSO was used as biological buffer in the following studies:
  • To maintain pH 9 during water leach test for the analysis of high-carbon coal fly ash-soil mixtures.
  • Chemiluminescent in situ hybridization using soybean peroxidase-labeled peptide nucleic acid (PNA) probes. I
  • Preparation of planar bilayer lipid membranes.
It may be employed as buffer for the electrophoretic analyses in non-denaturing gels, to investigate macromolecular self-association of ADP-ribosyltransferase protein in solution.

General description

CAPSO is a useful secondary standard buffer. Second dissociation constant pK2 value of CAPSO has been measured. Useful pH range for CAPSO is 8.9 - 10.3.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000499562
0000499562
0000499563
0000499563
WXBF5580V

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Second dissociation constants of 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS) and 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid (CAPSO) from the temperatures 278.15 K to 328.15 K.
Roy RN, et al.
The Journal of Chemical Thermodynamics, 29(7), 749-756 (1997)
P I Bauer et al.
The Biochemical journal, 270(1), 17-26 (1990-08-15)
The macromolecular self-association of ADP-ribosyltransferase protein in solution was studied by several experimental techniques: quantitative gel filtration, electrophoretic analyses in non-denaturing gels, and cross-linking the enzyme protein with glutaraldehyde, dimethyl pimelimidate, dimethyl suberimidate, dimethyl 3,3'-dithiobisproprionimidate and tetranitromethane. The self-association of
H Stender et al.
Applied and environmental microbiology, 67(1), 142-147 (2001-01-03)
A new chemiluminescent in situ hybridization (CISH) method provides simultaneous detection, identification, and enumeration of culturable Escherichia coli cells in 100 ml of municipal water within one working day. Following filtration and 5 h of growth on tryptic soy agar
Andreas Missner et al.
The Journal of biological chemistry, 283(37), 25340-25347 (2008-07-12)
Several membrane channels, like aquaporin-1 (AQP1) and the RhAG protein of the rhesus complex, were hypothesized to be of physiological relevance for CO(2) transport. However, the underlying assumption that the lipid matrix imposes a significant barrier to CO(2) diffusion was
Evaluation of leaching protocols for testing of high-carbon coal fly ash-soil mixtures.
Becker J, et al.
Journal of Environmental Engineering (New York, N.Y.), 139(5), 642-653 (2012)
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