C4187
Cyanoborohydride Coupling Buffer
Synonym(s):
Coupling buffer
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About This Item
NACRES:
NA.56
UNSPSC Code:
12161700
form
liquid
reaction suitability
reagent type: reductant
technique(s)
affinity chromatography: suitable
application(s)
life science and biopharma
storage temp.
2-8°C
Quality Level
Related Categories
Application
A ready-to-use reagent used to couple amine ligands to aldehyde functional groups. The coupling buffer reaction is a reductive amination of the intermediate Schiff′s base to a stable C−N bond.
Cyanoborohydride Coupling Buffer has been used:
- in coupling reactions between amines and glutaraldehyde
- to reduce hydrazone bond to a stable hydrazide bond
- as a component in oligonucleotide reaction mixture for coverslips functionalization
Cyanoborohydride Coupling Buffer is used in affinity chromatography, protein chromatography, activated/functionalized matrices and synthetic reagents. Cyanoborohydride has been used to inform a safe and effective gene-transfer system targeting hepatocytes as well as to develop a method for targeted delivery of anticancer therapeutics to cancer cells in hypoxic areas.
Biochem/physiol Actions
Cyanoborohydride Coupling Buffer is a reagent suitable for reductive amination processes, that contributes to transformation of simple alcohols into more complex amines. It is used in the conversion of Schiff base, by reducing it, to form a secondary amine without affecting aldehyde groups on the support.
Preparation Note
0.02 M sodium phosphate, pH 7.5, containing 0.2 M sodium chloride and 3.0 g/L sodium cyanoborohydride
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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