COS-7-InVitrus
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
African green monkey kidney
packaging
tube of 5 μg 05063001-DNA-5UG
pkg of vial of cells 05063001-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
receptors
Not specified
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Biochem/physiol Actions
COS-7 cell line (Sigma catalog no. 87021302) adapted to grow in the chemically defined protein- and peptide-free InVitrus medium (Cell Culture Technologies). This cell line is substrate dependent, i.e., adherent. COS-7 was derived from CV-1 simian cells transformed by an origin-defective mutant of SV40.
Monkey African green kidney, SV40 transformed, serum-free
Preparation Note
InVitrus medium (Cell Culture Technologies http://www.cellculture.com/) + 4 mM L-Glutamine (G7513) + 5 ug/ml Insulin. When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO (D2650).
Split sub-confluent cultures (70-80%) 1:2, i.e., seeding at 5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Please note: As the cell culture medium does not contain protein it will not inactivate trypsin. Once cells have detached from the flask it is necessary to dilute the trypsin with the cell culture medium, centrifuge the cells and resuspend in fresh medium. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.
Other Notes
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