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KARPAS 422

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Synonym(s):

KARPAS, 422

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About This Item

UNSPSC Code:
41106514

Key Documents

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biological source

human lymph (lymphoma)

packaging

tube of 5 μg 06101702-DNA-5UG
pkg of vial of cells 06101702-1VL

growth mode

Suspension

karyotype

Hyperdiploid with 10% polyploidy

morphology

Round polygonal cells growing singly or in small clusters in suspension

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

Biochem/physiol Actions

Established from a pleural effusion of 73 year-old woman diagnosed with B-cell non-Hodgkin lymphoma (intra-abdominal, diffuse large cell lymphoma, refractory, terminal). Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).
Human B cell non-Hodgkin lymphoma
Not specified

Preparation Note

Maintain cultures between 0.5 - 2 x 1000,000 cells/ml; 5% CO2; 37 °C. Doubling time approximately 60-90 hours. Split saturated culture 1:2 every 2-4 days. Round polygonal cells growing singly or in small clusters in suspension.
RPMI 1640 + 2 mM Glutamine + 20% Fetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Regulatory Information

监管及禁止进口产品

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