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About This Item
UNSPSC Code:
41106514
eCl@ss:
32160703
Biological source:
Mouse x Rat Glioma Hybrid brain
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Neuronal
Manufacturer/tradename:
ECACC
Product Name
NH15-CA2, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
Mouse x Rat Glioma Hybrid brain
manufacturer/tradename
ECACC
packaging
tube of 5 μg 08062525-DNA-5UG, pkg of vial of cells 08062525-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
Acetylcholine and noradrenaline
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
used as a neuronal model
Biochem/physiol Actions
Mouse neuroblastoma x rat glioma hybrid
NH15-CA2 is a hybrid cell line formed by the fusion of N115-BU-8 (a bromodeoxyuridine-resistant mutant of N1E-115 (Sigma Catalogue number 08062519) and 108CC15 (Sigma Catalogue number 08062516) (itself a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid line). This “hybrid-hybrid” line contains both cholinergic and adrenergic enzyme activities.
Preparation Note
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 days
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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