C6-BU-1
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
rat brain
packaging
tube of 5 μg 08062563-DNA-5UG
pkg of vial of cells 08062563-1VL
growth mode
Adherent
karyotype
modal No. of chromosomes is 42
morphology
fibroblastic/fusiform
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Used in the generation of cell hybrids.
Biochem/physiol Actions
A 8-bromodeoxybromidine resistant mutant of the rat glioma cell line C6. Found to be tumourigenic in BALB/c nude mice and Wistar rats. Strain rattus norvegicus
Rat glioma x rat glioma hybrid
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine.
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C
Other Notes
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