Mero-14

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Mero-14 was derived from the pleural effusion from a male patient aged >60 with a history of asbestos exposure. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18

Hams F10 + 15% FCS + 2mM Glutamine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 24hrs

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.