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Mero-14

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 9100101, human lung, Spindle shaped cells

Synonym(s):

Mero14 Cells

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About This Item

UNSPSC Code:
41106514

Key Documents

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biological source

human lung

packaging

tube of 5 μg 09100101-DNA-5UG
pkg of vial of cells 09100101-1VL

growth mode

Adherent

karyotype

Number of chromosomes 72-75

morphology

Spindle shaped cells

products

Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Biochem/physiol Actions

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure
Mero-14 was derived from the pleural effusion from a male patient aged >60 with a history of asbestos exposure. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18

Preparation Note

Hams F10 + 15% FCS + 2mM Glutamine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 24hrs

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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