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About This Item
UNSPSC Code:
41106514
Biological source:
mouse adipose tissue
Growth mode:
Adherent
Karyotype:
Normal diploid
Morphology:
Fibroblast-like
biological source
mouse adipose tissue
packaging
tube of 5 μg 11112201-DNA-5UG, pkg of vial of cells 11112201-1VL
growth mode
Adherent
karyotype
Normal diploid
morphology
Fibroblast-like
receptors
Sca-1, c-kit/CD117, nestin, nucleostemin, CD44 and CD106
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Biochem/physiol Actions
Adipose cell line, mouse subcutaneous, mesenchymal, multipotent
Not specified
m17.ASC is a spontaneously immortalized multipotent mesenchymal cell line capable of acquiring adipocyte, chondrocyte, osteocyte and cardiomyocyte phenotypes when cultured with appropriate differentiation factors. m17.ASC provides a valuable tool for in vitro biomimetic studies; assessing growth technologies such as 3-D structures, biochemical cues (selected growth factors and hormones) and physical forces (electrical, mechanical and topographical). It can be used to screen compounds that induce or inhibit proliferation and/or differentiation in tissue specific phenotypes.
Preparation Note
Split sub-confluent cultures (90%) approximately 1:6 with media changes or subculture every 2-3 days seeding at 0.5-4 x 104 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
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Regulatory Information
低风险生物材料
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